Response of a spontaneous murine tumor to hyperthermia: factors which modify the thermal response in vivo.
Response of a spontaneous murine tumor to hyperthermia: factors which modify the thermal response in vivo.
复制标题
自发性鼠肿瘤对高热的反应：改变体内热反应的因素。
DOI：
10.2307/3575282
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发表时间：
1980
影响因子：
3.4
通讯作者：
H. Suit
中科院分区：
文献类型：
--
作者：
M. Urano;L. Gerweck;R. Epstein;M. Cunningham;H. Suit
本文研究了影响C3Hf/SED小鼠自发性纤维肉瘤热反应的因素。在43.5℃恒温水浴中，通过浸泡小鼠脚或培养瓶进行热疗，第一次实验证实了pH效应。体外培养的单细胞悬液在不同pH条件下处理，用肺集落形成法检测细胞存活。存活曲线的斜率随pH水平的降低而减小，外推值不变。为了研究肿瘤大小对热响应的影响，对四种不同大小的肿瘤进行了体内治疗。肿瘤生长分析表明，热敏感性的增加与肿瘤体积的增大有关。在一半的治疗动物中，达到肿瘤控制的治疗时间TCD5也随着肿瘤体积的增加而减少，即35、100、270和800 mm~3的肿瘤分别为170、141、123和111分钟。体内和体外细胞存活的对比研究强烈表明，肿瘤的pH值随着肿瘤的生长而降低。试图通过在呼吸空气中添加15%的二氧化碳来降低血液pH值；这导致了热反应的轻微增强。对这些结果进行了讨论。
Factors which may modify the thermal response of a C3Hf/Sed mouse spontaneous fibrosarcoma were studied. Hyperthermia was given in a constant-temperature water bath by immersing the mouse foot or culture flask at 43.5'C. The pH effect was confirmed in the first experiment. Single-cell suspensions were treated at various pH in vitro and cell survival was determined by lung colony assay in vivo. The slope of the survival curve decreased with decreasing pH level without changing extrapolation numbers. To study the effect of tumor size on the thermal response, tumors of four different sizes were treated in vivo. Tumor growth analysis demonstrated that an increase of the thermal sensitivity was associated with increasing tumor volume. TCD5s, treatment time to achieve a tumor control in half the treated animals, also decreased with increasing tumor volume, i.e., 170, 141, 123, and 111 min for 35-, 100-, 270-, and 800-mm3 tumors, respectively. Comparative studies on in vivo and in vitro cell survival strongly suggested that the tumor pH decreases with tumor growth. An attempt was made to reduce blood pH by adding 15% CO2 in the respiratory air; this resulted in slight enhancement of the thermal response. These results are discussed.