Hematopoietic and nature killer cell development from human pluripotent stem cells.
Hematopoietic and nature killer cell development from human pluripotent stem cells.
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DOI：
10.1007/978-1-62703-478-4_3
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Kaufman, Dan S
中科院分区：
文献类型：
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作者：
Ni, Zhenya;Knorr, David A;Kaufman, Dan S
自然杀伤 (NK) 细胞是先天免疫系统的关键效应细胞，可保护宿主免受各种感染以及恶性细胞的侵害。 NK 细胞生物学领域的最新进展使人们更好地了解 NK 细胞如何发育。这一进展直接改善了接受造血干细胞移植治疗潜在致命恶性肿瘤的患者的预后。然而，小鼠和人类 NK 细胞发育和生物学之间的关键差异限制了利用啮齿动物来更深入地了解 NK 细胞发育。因此，需要一种易于获取且遗传上易于处理的细胞来源来研究人类 NK 细胞的发育。我们的实验室率先从人类胚胎干细胞 (hESC) 和最近的诱导多能干细胞 (iPSC) 中开发淋巴细胞，特别是 NK 细胞。本章介绍了一种使用鼠基质细胞系从 hESC 和 iPSC 生成 NK 细胞的可靠方法。此外，我们还采用了一种使用旋转胚体 (spin-EB) 分化系统的更新方法，该系统允许人类 NK 细胞在体外条件下完全发育。
Natural killer (NK) cells are key effectors of the innate immune system, protecting the host from a variety of infections, as well as malignant cells. Recent advances in the field of NK cell biology have led to a better understanding of how NK cells develop. This progress has directly translated to improved outcomes in patients receiving hematopoietic stem cell transplants to treat potentially lethal malignancies. However, key differences between mouse and human NK cell development and biology limits the use of rodents to attain a more in depth understanding of NK cell development. Therefore, a readily accessible and genetically tractable cell source to study human NK cell development is warranted. Our lab has pioneered the development of lymphocytes, specifically NK cells, from human embryonic stem cells (hESCs) and more recently induced pluripotent stem cells (iPSCs). This chapter describes a reliable method to generate NK cells from hESCs and iPSCs using murine stromal cell lines. Additionally, we include an updated approach using a spin-embryoid body (spin-EB) differentiation system that allows for human NK cell development completely defined in vitro conditions.