Thermo-responsive polymer brushes on glass plate prepared from a new class of amino acid-derived vinyl monomers and their applications in cell-sheet engineering
Thermo-responsive polymer brushes on glass plate prepared from a new class of amino acid-derived vinyl monomers and their applications in cell-sheet engineering
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DOI：
10.1016/j.colsurfb.2017.07.068
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发表时间：
2017-11-01
影响因子：
5.8
通讯作者：
Koga, Tomoyuki
中科院分区：
文献类型：
--
作者：
Higashi, Nobuyuki;Hirata, Ayaha;Koga, Tomoyuki
在这项研究中，我们提出了一种新的温度响应性聚合物平台，这是基于丙氨酸甲基酯的均聚物（PNAAMe）和共聚物与甘氨酸甲基酯基乙烯基单体（P（NAAMe-co-NAGMe））刷通过表面引发的原子转移自由基聚合制备。在不同温度下测量的这些刷的水接触角揭示了聚合物刷在升高温度时分别在13摄氏度和25摄氏度（Trs）左右塌陷和破裂。在37摄氏度下，接种的成纤维细胞（NIH/3 T3）粘附并良好地扩散到这些刷表面上，尽管P（NAAMe-co-NAGMe）的共聚物刷在24小时后使粘附细胞的数量减少到PNAAMe均聚物的一半以下。由于亲水性的增加，细胞培养24小时。为了制备细胞片层，将细胞接种在两种聚合物刷上，并在血清存在下于37 ° C下培养。4天后，细胞在这些刷表面上汇合增殖。将温度降低至每个刷的TT以下4 ° C和20 ° C，导致细胞片以单层形式从聚合物刷脱离，伴随着表面亲和力的转换。(C)2017爱思唯尔B. V.保留所有权利。
In this study, we present a novel thermo-responsive polymer platform that is based on the alanine methyl ester-containing homopolymer (PNAAMe) and the copolymer with glycine methyl ester-based vinyl monomer (P(NAAMe-co-NAGMe)) brushes prepared via surface-initiated atom transfer radical polymerization. Water contact angles for these brushes measured at different temperatures reveal that the polymer brushes collapse and dehydrate around 13 degrees C and 25 degrees C (Trs), respectively, upon elevating the temperature. At 37 degrees C, seeded fibroblasts (NIH/3T3) adhere to and spread well onto these brush surfaces although the copolymer brush of P(NAAMe-co-NAGMe) depresses the number of adherent cells less than half of that for the homopolymer of PNAAMe after 24 h of cell culture due to increment in hydrophilicity. To prepare the cell-sheet, the cells are seeded on both polymer brushes and cultured at 37 degrees C in the presence of serum. After 4 days, the cells proliferated confluently on these brush surfaces. Lowering the temperature to 4 degrees C and 20 degrees C below TT of each brush led to the cell-sheet detachment as a monolayer form from the polymer brushes accompanying with the switching of surface affinity. (C) 2017 Elsevier B.V. All rights reserved.