PHOSPHATIDYLETHANOL FORMATION VIA TRANSPHOSPHATIDYLATION BY RAT-BRAIN SYNAPTOSOMAL PHOSPHOLIPASE-D
PHOSPHATIDYLETHANOL FORMATION VIA TRANSPHOSPHATIDYLATION BY RAT-BRAIN SYNAPTOSOMAL PHOSPHOLIPASE-D
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DOI：
10.1111/j.1471-4159.1987.tb05707.x
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发表时间：
1987-05-01
影响因子：
4.7
通讯作者：
KANFER, JN
中科院分区：
文献类型：
--
作者：
KOBAYASHI, M;KANFER, JN
在大鼠脑突触体富集制剂中，证实了磷脂酶D转脂活性所催化的磷脂酰乙醇(PEH)生成。确定最适pH为6.5，最佳乙醇浓度为0.3-0.4M，表观Km值为0.2M，在没有合适激活剂的情况下，几乎检测不到Peth的生成，发现几种不饱和脂肪酸是有效的激活剂。最大激活所需的油酸浓度随孵育混合物中内源性磷脂酰胆碱的浓度而变化。所有被测试的洗涤剂的活性都明显低于不饱和脂肪酸，并且不需要二价离子来形成聚乙醇胺。磷脂酰胆碱是受试磷脂中最有效的磷脂供体。在所研究的不同脑亚组分中，突触膜组分的PEH形成活性最强。肺、心脏和脂肪组织中12,000克-100,000克颗粒物的活性与大脑相似。
Phosphatidylethanol (Peth) formation catalyzed by the transphospatidylation activity of phospholipase D was demonstrated to occur in a rat brain synaptosomal enriched preparation. The optimal pH was determined to be 6.5, and the optimal ethanol concentration was determined to be 0.3-0.4 M with an apparent Km of 0.2 M. Peth formation was barely detectable in the absence of an appropriate activator and several unsaturated fatty acids were found to be effective activators. The concentrations of oleic acid required for maximum activation varied with the concentration of endogenous phosphatidylcholine present in the incubation mixtures. All detergents tested were significantly less active than the unsaturated fatty acids and divalent ions were not required for Peth formation. Phosphatidylcholine was the most effective phosphatidyl donor of the phospholipids tested. Peth forming activity was greatest in the synaptic membrane fraction of the various brain subfractions examined. The 12,000 g-100,000 g particulate fraction of lung, heart, and adipose tissue had activities similar to that of brain.