A new technique of in vivo synchrotron radiation coronary microangiography in the rat
A new technique of in vivo synchrotron radiation coronary microangiography in the rat
复制标题
大鼠体内同步辐射冠状动脉显微血管造影新技术
DOI：
10.1177/0284185114549570
复制
发表时间：
2015
期刊：
影响因子：
1.3
通讯作者：
Y. Hiramatsu
中科院分区：
文献类型：
--
作者：
H. Sakamoto;S. Matsushita;K. Hyodo;C. Tokunaga;F. Sato;Y. Hiramatsu
背景以前，在我们的实验室中，使用朗宁多夫灌流的大鼠心脏的同步辐射冠状动脉微血管造影术可以显示直径50 微米的冠状动脉。然而，在体内，由于大鼠的心率过快，SRCA带来了时间分辨率下降的问题。目的建立静脉注射三磷酸腺苷二钠(ATP)诱发大鼠在体心动过缓的简便方法。材料与方法在高能加速器研究机构(日本筑波)的光子工厂进行SRCA。雄性Wistar大鼠8只，麻醉。将注射造影剂的导管插入颈动脉。静脉推注5 mg三磷酸腺苷诱发暂时性心动过缓，术后即刻行自发性冠状动脉腔内成形术。结果服药后平均心率由388次/min降至73次/min。结果，我们可以检测到直径小至45 微米的冠状动脉。结论该系统具有9 /像素的高分辨率，可检测到小至45 µm的在体大鼠冠状动脉。
Background Previously, in our laboratory, synchrotron radiation coronary microangiography (SRCA) using Langendorff-perfused rat hearts could visualize a coronary artery of 50 µm in diameter. However, in vivo rat SRCA poses the problem of compromised temporal resolution due to the rapid heart rate of rats. Purpose To establish a simple method of in vivo rat SRCA with bradycardia induced by intravenous injection of adenosine triphosphate disodium hydrate (ATP). Material and Methods SRCA was performed at the Photon Factory of the High Energy Accelerator Research Organization (Tsukuba, Japan). Eight male Wistar rats were anesthetized. A catheter for injecting the contrast material was inserted into the carotid artery. Temporary bradycardia was induced by an intravenous bolus injection of 5 mg of ATP, and SRCA was performed immediately thereafter. Results After ATP administration, the average heart rate decreased from 388 to 73 beats per minute. As a result, we could detect a coronary artery as small as 45 µm in diameter. Conclusion Our SRCA system which has a high resolution of 9 µm per pixel could detect a coronary artery as small as 45 µm in diameter in the in vivo rat.
外周动脉对寒冷暴露反应的性别差异
DOI：
--
发表时间：
2008
期刊：
Circulation Journal 72(in press)
影响因子：
--
作者：
Sato F;Sakakibara Y;et. al.
通讯作者：
et. al.