Characterization of gamma-aminobutyric acid and dopamine overflow following acute implantation of a microdialysis probe.
Characterization of gamma-aminobutyric acid and dopamine overflow following acute implantation of a microdialysis probe.
复制标题
微透析探针急性植入后γ-氨基丁酸和多巴胺溢出的表征。
DOI：
10.1016/0024-3205(89)90134-3
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发表时间：
1989
期刊：
影响因子：
6.1
通讯作者：
Ungerstedt,U
中科院分区：
文献类型：
--
作者：
Drew,KL;O'Connor,WT;Kehr,J;Ungerstedt,U
本研究的特点是电压和钙依赖性的γ-氨基丁酸和多巴胺溢出后，急性植入微透析探针。将探针植入背外侧纹状体和苍白球。实验在轻度氟烷麻醉下进行。GABA的基础，细胞外水平不受河豚毒素（TTX）的影响，并增加到140%的基础值的无钙林格氏。基础，细胞外多巴胺水平降低到基础值的14%，通过添加TTX和基础值的30%，通过从林格溶液中除去钙。结果表明，在这种体内制备基础细胞外多巴胺主要是囊泡起源，而GABA不是。
The present study characterized the voltage and calcium dependence of gamma-aminobutyric acid and dopamine overflow after the acute implantation of a microdialysis probe. Probes were implanted in dorsolateral striatum and globus pallidus. Experiments were performed under light halothane anesthesia. Basal, extracellular levels of GABA were not affected by tetrodotoxin (TTX) and were increased to 140 percent of basal values by calcium free Ringer. Basal, extracellular levels of dopamine were reduced to 14 percent of basal values by the r addition of TTX and to 30 percent of basal values by the removal of calcium from the Ringer solution. The results suggest that in this in vivo preparation basal extracellular dopamine is largely of vesicular origin while GABA is not.