Editing through multiple bonds: Threonine detection
Editing through multiple bonds: Threonine detection
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DOI：
10.1002/mrm.21492
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发表时间：
2008-02-01
影响因子：
3.3
通讯作者：
Gruetter, Rolf
中科院分区：
文献类型：
--
作者：
Marjanska, Malgorzata;Henry, Pierre-Gilles;Gruetter, Rolf
在体内H-1光谱中，1.32 ppm处的信号通常归属于乳酸盐。该共振位置在生理pH下与苏氨酸共享。乳酸盐和苏氨酸的光谱模式的相似性使得在没有编辑的情况下甚至在编辑的情况下单独测量苏氨酸或乳酸盐具有技术挑战性。在这项研究中，使用单发多键编辑技术检测苏氨酸信号，并在大鼠和人脑中进行体内定量。估计大鼠脑中的苏氨酸浓度为0.8 +/- 0.3 mM（平均值+/- SD，n = 6），人脑中的苏氨酸浓度与0.33 mM相似。
In in vivo H-1 spectroscopy, the signal at 1.32 ppm is usually assigned to lactate. This resonance position is shared with threonine at physiological pH. The similarity of spectral patterns of lactate and threonine renders the separate measurement of either threonine or lactate without and even with editing technically challenging. In this study, the threonine signal was detected using a single-shot multiple-bond editing technique and quantified in vivo in both rat and human brains. A threonine concentration was estimated at 0.8 +/- 0.3 mM (mean +/- SD, n = 6) in the rat brain and at similar to 0.33 mM in the human brain.