The effect of fluoride on the resorption of dentine by osteoclasts In Vitro
The effect of fluoride on the resorption of dentine by osteoclasts In Vitro
复制标题
氟化物对破骨细胞体外吸收牙本质的影响
DOI：
10.1002/jbmr.5650051391
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发表时间：
1990
影响因子：
6.2
通讯作者：
Sheila J. Jones
中科院分区：
文献类型：
--
作者：
M. L. Taylor;E. Maconnachie;K. Frank;A. Boyde;Sheila J. Jones
从孵化前雏鸡的骨骼中机械分离破骨细胞，在不加氟化钠或添加0.15、1、15、30 mg/L氟化钠的培养液中，用牙本质培养24或39h。对固定标本和甲苯胺蓝染色标本进行光镜观察，计数破骨细胞，取出细胞，计数吸收陷窝。然后，牙本质被准备用于扫描电子显微镜(SEM)，并使用背散射电子成像在栅格搜索上定位凹坑。进行了立体摄影测量，测量了坑的深度、平面和真实表面积以及体积，并计算了每个坑的体积：面积比和三维形状系数。记录了每个矿坑周围有无脱矿环。在含15或30 mg/L NaF的培养中，相同平面面积的凹坑深度显著减少(p<；0.001)，坑周围环状的发生率较高(对照组分别为2.7%、15 mg/L 313%、30 mg/L 39.7%)。在一项实验中，添加1毫克/升氟化钠的培养标本中的凹坑更大，但在一个更大的系列631个(对照组为251个，1 mg/升NaF培养组为380个)中，测量的凹坑的深度、体积和体积：平面面积比显著降低(p<；0.001)，环状凹坑的发生率是氟处理组的2.5%。在这种氟浓度下，对培养中某些细胞的刺激和对破骨细胞的间接刺激以及对破骨细胞吸收功能的直接抑制的复杂相互作用可能正在发挥作用。在体内，治疗剂量的氟化钠预计不仅会增加类骨质的形成，还会抑制破骨活性。
Osteoclasts were isolated mechanically from bones of prehatch chicks and cultured on dentine for 24 or 39 h in medium without or with added sodium fluoride at concentrations of 0.15, 1, 15, or 30 mg/liter. Fixed and toluidine blue‐stained specimens were examined by light microscopy and osteoclasts were counted, all cells were removed and resorption pits were counted. The dentine was then prepared for scanning electron microscopy (SEM) and pits located on a raster search using backscattered electron imaging. Stereophotogrammetric measurements were made of the depths, plan and true surface areas and volumes of pits, and the volume: area ratio and a three‐dimensional form factor calculated for each. The presence or absence of a ring of demineralization around each pit was recorded. Pits with the same plan area were significantly (p < 0.001) less deep in cultures containing 15 or 30 mg/liter NaF and the incidence of rings around pits was higher (controls 2.7%, 15 mg/liter 313%, 30 mg/liter NaF 39.7%). In one experiment, pits were larger in the specimen cultured with added 1 mg/liter NaF, but in a much larger series of 631 (251 in the control and 380 in the 1 mg/liter NaF cultures) pits measured, depths, volumes and volume:plan‐area ratios were significantly lower (p < 0.001), and rings round pits 2.5 times more common, in the fluoride‐treated group. A complex interplay of stimulation of some cells in the culture with indirect stimulation of osteoclasts and direct inhibition of osteoclastic resorptive function may be operating at this concentration of fluoride. In vivo, therapeutic doses of sodium fluoride would be expected to act not only to increase the formation of osteoid but also to suppress osteoclastic activity.