Analysis of Th1/Th2 response pattern for erythrodermic psoriasis
Analysis of Th1/Th2 response pattern for erythrodermic psoriasis
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红皮病型银屑病Th1/Th2反应模式分析
DOI：
10.1007/s11596-014-1322-0
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发表时间：
2014-08-01
影响因子：
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通讯作者：
Tu, Ya-ting
中科院分区：
文献类型：
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作者：
Zhang, Ping;Chen, Hong-xiang;Tu, Ya-ting
红皮病型银屑病（erythrodermic psoriasis，EP）是银屑病最严重的类型之一，其发病机制至今尚不清楚。本研究旨在检测EP患者外周血单个核细胞（PBMCs）中Th 1/Th 2细胞因子、T淋巴细胞克隆水平及皮损组织中T-bet/加塔-3基因表达水平。本文还对Th 1/Th 2反应模式在EP发病机制中的作用进行了讨论。采用酶联免疫吸附试验（ELISA）检测16例EP患者、20例寻常型银屑病（PV）患者和15例健康对照者血清中IFN-γ、IL-2、IL-4和IL-10水平。通过实时定量PCR检测皮损和PBMC中T-bet/加塔-3的表达水平。流式细胞仪检测Th 1/Th 2比值。EP患者血清IFN-γ、IL-2、IL-4和IL-10水平均高于健康对照组。EP组IL-4和IL-10水平分别为69.44±11.45和12.62± 4.57pg/mL，显著高于PV组和健康对照组（P<0.05）。流式细胞仪检测结果显示，EP患者外周血Th 1、Th 2细胞水平均高于正常对照组，Th 1/Th 2比值低于PV患者（P<0.01）。EP组IFN-γ/IL-4和T-bet/加塔-3比值均小于1.0，与其他两组相比，有逆转作用。提示EP患者存在Th 1/Th 2双向反应模式，Th细胞亚群平衡向Th 2型倾斜，这可能是EP发病的重要机制之一。
As one of the most serious types of psoriasis, pathogenesis of erythrodermic psoriasis (EP) is unclear so far. In this study, we aimed to detect the levels of Th1/Th2 cytokine-associated transcription factors and T-lymphocyte clone in peripheral blood mononuclear cells (PBMCs) derived from EP patients, and gene expression level of T-bet/GATA-3 in skin lesion. The potential role of Th1/Th2 reaction pattern played in the pathogenesis of EP was also discussed. Serum levels of IFN-γ, IL-2, IL-4 and IL-10 were quantified by ELISA among 16 EP patients, 20 psoriasis vulgaris (PV) patients and 15 healthy controls. The expression levels of T-bet/GATA-3 in the skin lesion and PBMCs were examined by real-time qPCR. The ratio of Th1/Th2 was measured by flow cytometry. The levels of IFN-γ, IL-2, IL-4 and IL-10 were higher in EP patients than in the healthy controls. The levels of IL-4 and IL-10 were 69.44±11.45 and 12.62±4.57 pg/mL, respectively, in EP patients, significantly higher than those in PV patients and healthy controls (P<0.05). Flow cytometry revealed the levels of both Th1 and Th2 in PBMCs from EP patients were higher than those in healthy controls, and the Th1/Th2 ratio was dramatically lower than in PV patients (P<0.01). The ratios of IFN-γ/IL-4 and T-bet/GATA-3 in EP patients were both less than 1.0, suggesting a reversal when compared with the other two groups. Our study indicated that the EP patients exerted a Th1/Th2 bidirectional response pattern, and the balance of Th cell subsets inclines to Th2, which might be one of the important mechanisms of EP pathogenesis.