Increased numbers of mast cells in the hyperplastic buccal mucosa of the zinc-deficient rat.
Increased numbers of mast cells in the hyperplastic buccal mucosa of the zinc-deficient rat.
复制标题
缺锌大鼠增生性颊粘膜中肥大细胞数量增加。
DOI：
10.1111/j.1600-0714.1981.tb01244.x
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Waterhouse,JP
中科院分区：
文献类型：
--
作者：
Kreavich,ME;Meyer,J;Waterhouse,JP
六个断奶雄性Sprague道利大鼠喂养的饮食中含有0.4 ppm的锌和7个被喂食相同的饮食，除了40 ppm的锌。4周后，取出面向磨牙区域的颊粘膜标本。将石蜡切片（切割为6 μ）用甲苯胺蓝染色，并对每只动物的5个切片进行描记，间隔不小于60 μm。在50 μm宽的固有层浅区和250 μ m宽的深区，对测量长度的切片进行肥大细胞计数，实验动物上皮下区的肥大细胞平均数为15.4个/mm，范围为9.2-33.1。对照组的平均值为4.0;范围为2.9-5.3。深层未见增加，上皮角化不全，厚度增加2倍。在切片的周边部分，细胞和角蛋白层的厚度均匀增加，但在中心部分，角蛋白层的厚度不成比例地增加了近4倍，细胞层的厚度增加较少。
Six weanling male Sprague Dawley rats were fed a diet containing 0.4 ppm Zn and seven were fed an identical diet except for 40 ppm Zn. After 4 weeks, specimens of buccal mucosa in the region facing the molar teeth were removed. Paraffin sections, cut at 6 μ, were stained with toluidine blue, and tracings made of five sections per animal, spaced no less than 60 μm apart. Counts of mast cells over a measured length of section were made in a superficial zone of the lamina propria of 50 μm width and a deeper zone of 250 μ width.The average number of mast cells per mm in the subepithelial zone of the experimental animals was 15.4, the range 9.2–33.1. The control average was 4.0; the range was 2.9–5.3. No increase was found in the deeper zone.The epithelium was parakeratotic and its thickness was increased two‐fold. In the peripheral portion of the section, cellular and keratin layers were evenly increased in thickness, but in the central portion a disproportionate, nearly four‐fold increase occurred in the keratin layer and a lesser increase in the cellular layer.