Multi-unit recording with iridium oxide modified stereotrodes in Drosophila melanogaster
Multi-unit recording with iridium oxide modified stereotrodes in Drosophila melanogaster
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DOI：
10.1016/j.jneumeth.2013.11.013
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发表时间：
2014-01
影响因子：
3
通讯作者：
Cheng Zhong;Yuanyuan Zhang;Wei He;Pengfei Wei;Yi Lu;Y. Zhu;Li Liu;Liping Wang
中科院分区：
文献类型：
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作者：
Cheng Zhong;Yuanyuan Zhang;Wei He;Pengfei Wei;Yi Lu;Y. Zhu;Li Liu;Liping Wang
果蝇是神经科学研究中一种非常有利的动物模型。然而，由于果蝇大脑体积较小，利用电生理方法研究果蝇体内神经元组装动力学仍然是一个巨大的挑战。人们非常需要用于多单元记录的小而灵敏的微电极。新方法制备了微尺度立体电极，用于黑腹果蝇的电记录。在高度可控的沉积过程中，用氧化铱（IrO2）修饰立体电极，以改善其电化学性能。使用iro2立体电极进行电记录，以检测体内自发动作电位和lfp。结果iro2电极在1khz时表现出较高的电容和较低的阻抗。iro2立体电极在体内的电记录显示，平均信噪比（SNR）为7.3，LFP灵敏度显著提高。5种不同类型的神经元记录清晰分离。对视觉和气味刺激的电生理反应也分别被检测到。在果蝇中，最广泛使用的电记录电极是玻璃微电极和锐化钨微电极，它们通常用于单单元记录。虽然四极体技术已被用于记录果蝇的多神经元活动，但制备的iro2立体电极具有更小的几何尺寸，但具有相当的记录信噪比和更好的分类质量。结论iro2立体电极能够满足多单元记录和脉冲分选的要求，将为基于电生理的研究，特别是对果蝇和其他小动物的电生理研究提供一个有用的工具。
BackgroundDrosophilais a very favorable animal model for the studies of neuroscience. However, it remains a great challenge to employ electrophysiological approaches inDrosophilato study the neuronal assembly dynamicsin vivo, partially due to the small size of theDrosophilabrain. Small and sensitive microelectrodes for multi-unit recordings are greatly desired.New methodWe fabricated micro-scale stereotrodes for electrical recordings inDrosophila melanogaster. The stereotrodes were modified with iridium oxide (IrO2) under a highly controllable deposition procedure to improve their electrochemical properties. Electrical recordings were carried out using the IrO2stereotrodes to detect spontaneous action potentials and LFPsin vivo.ResultsThe IrO2electrodes exhibited significantly higher capacitance and lower impedance at 1 kHz. Electrical recording with the IrO2stereotrodesin vivodemonstrated an average signal-to-noise ratio (SNR) of 7.3 and a significantly improved LFP sensitivity. 5 types of different neurons recorded were clearly separated. Electrophysiological responses to visual and odor stimulation were also detected, respectively.Comparison with existing method(s)The most widely used electrodes for electrical recording inDrosophilaare glass microelectrode and sharpened tungsten microelectrode, which are typically used for single-unit recordings. Although tetrode technology has been used to record multi-neuronal activities fromDrosophila, the fabricated IrO2stereotrodes possess smaller geometry size but exhibited comparable recording signal-to noise ration and better sorting quality.ConclusionsThe IrO2stereotrodes are capable to meet the requirements of multi-unit recording and spike sorting, which will be a useful tool for the electrophysiology-based researches especially inDrosophilaand other small animals.