IDENTIFICATION OF LACTATE DEHYDROGENASE-ELEVATING VIRUS AS THE ETIOLOGICAL AGENT OF GENETICALLY RESTRICTED, AGE-DEPENDENT POLIOENCEPHALOMYELITIS OF MICE
IDENTIFICATION OF LACTATE DEHYDROGENASE-ELEVATING VIRUS AS THE ETIOLOGICAL AGENT OF GENETICALLY RESTRICTED, AGE-DEPENDENT POLIOENCEPHALOMYELITIS OF MICE
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DOI：
10.1128/iai.27.3.979-987.1980
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发表时间：
1980-01-01
影响因子：
3.1
通讯作者：
PHELPS, AH
中科院分区：
文献类型：
--
作者：
MARTINEZ, D;BRINTON, MA;PHELPS, AH
采用生物学、理化和抗原标准对遗传限制性、年龄依赖性小鼠脊髓灰质炎脑脊髓炎（ADPE）病原和几种乳酸脱氢酶升高病毒（LDV）分离株的病原进行了比较。ADPE制剂可能是一株LDV。与LDV一样，ADPE可诱导小鼠血浆酶选择性升高和脾肿大。ADPE制剂的促酶活性和致麻痹活性属于同一病毒。ADPE在体内和体外均表现出类似ldv的复制动力学。ADPE制剂需要小鼠原代巨噬细胞进行体外复制，LDV也是如此。LDV分离株在免疫抑制的C58小鼠脊髓中诱导具有adpe样病变的麻痹性疾病。与ADPE相比，LDV分离株对小鼠麻痹的依赖性更强。LDV分离物和ADPE试剂在形态、浮力密度、结构蛋白模式和病毒粒子RNA沉降速率方面没有区别。它们表现出很强的抗原交叉反应性，这是通过体内交叉保护和放射免疫测定确定的。
The etiological agent of genetically restricted, age-dependent polioencephalomyelitis of mice (the ADPE agent) and several isolates of lactate dehydrogenase-elevating virus (LDV) were compared by biological, physical-chemical and antigenic criteria. The ADPE agent may be a strain of LDV. Like LDV the ADPE agent induced a selective elevation of plasma enzymes and splenomegaly in mice. The enzyme-elevating activity and the paralytogenic activity of the ADPE agent preparations belonged to the same virus. The ADPE agent demonstrated LDV-like replication kinetics in vivo and in vitro. The ADPE agent required primary mouse macrophages for in vitro replication, as does LDV. The LDV isolates induced a paralytic disease with ADPE-like lesions in the spinal cords of immunosuppressed C58 mice. LDV isolates showed stronger dependence on strain and age of mouse for the induction of paralysis than did the ADPE agent. LDV isolates and the ADPE agent exhibited indistinguishable morphologies, buoyant densities, structural protein patterns and virion RNA sedimentation rates. They displayed strong antigenic cross-reactivity, as determined by cross-protection in vivo and by radioimmunoassay.