Carbohydrate-related enzymes of important Phytophthora plant pathogens
Carbohydrate-related enzymes of important Phytophthora plant pathogens
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DOI：
10.1016/j.fgb.2014.08.011
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发表时间：
2014-11-01
影响因子：
3
通讯作者：
de Vries, Ronald P.
中科院分区：
文献类型：
--
作者：
Brouwer, Henk;Coutinho, Pedro M.;de Vries, Ronald P.
碳水化合物活性酶（CAZymes）形成了植物病原体研究中特别有趣的目标。尽管许多 CAZymes 是致病因子，但文献中卵菌 CAZymes 受到的关注明显少于效应因子。在这里，我们对致病疫霉、Ph. ramorum、Ph. sojae 和终极腐霉基因组中存在的 CAZymes 进行了分析，并与这些物种在一系列不同碳源上的生长进行了比较。在这些碳源上的生长表明，参与细胞壁相关底物（如纤维素、木聚糖和果胶）降解的酶家族的大小并不总是这些底物上生长的良好预测因子。虽然存在降解木聚糖和纤维素的能力，但产品并未完全糖化并用作碳源。与 Py 相比，疫霉菌基因组编码更大的 CAZyme 集。 ultimum，并编码 Py 中缺失的假定角质酶、GH12 木葡聚糖酶和 GH10 木聚糖酶。终极基因组。疫霉属还编码大量参与果胶降解的酶家族和基因。在疫霉菌基因组之间没有发现完整酶家族的损失或增加，但某些酶家族的大小存在一些显着差异。 (C) 2014 Elsevier Inc. 保留所有权利。
Carbohydrate-Active enZymes (CAZymes) form particularly interesting targets to study in plant pathogens. Despite the fact that many CAZymes are pathogenicity factors, oomycete CAZymes have received significantly less attention than effectors in the literature. Here we present an analysis of the CAZymes present in the Phytophthora infestans, Ph. ramorum, Ph. sojae and Pythium ultimum genomes compared to growth of these species on a range of different carbon sources. Growth on these carbon sources indicates that the size of enzyme families involved in degradation of cell-wall related substrates like cellulose, xylan and pectin is not always a good predictor of growth on these substrates. While a capacity to degrade xylan and cellulose exists the products are not fully saccharified and used as a carbon source. The Phytophthora genomes encode larger CAZyme sets when compared to Py. ultimum, and encode putative cutinases, GH12 xyloglucanases and GH10 xylanases that are missing in the Py. ultimum genome. Phytophthora spp. also encode a larger number of enzyme families and genes involved in pectin degradation. No loss or gain of complete enzyme families was found between the Phytophthora genomes, but there are some marked differences in the size of some enzyme families. (C) 2014 Elsevier Inc. All rights reserved.