Characterization of the Aspergillus nidulans septin (asp) gene family.
Characterization of the Aspergillus nidulans septin (asp) gene family.
复制标题
构巢曲霉 septin (asp) 基因家族的特征。
DOI：
10.1093/genetics/157.3.969
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发表时间：
2001
期刊：
影响因子：
3.3
通讯作者：
Westfall,PJ
中科院分区：
文献类型：
--
作者：
Momany,M;Zhao,J;Lindsey,R;Westfall,PJ
Septin基因家族的成员参与胞质分裂和组织生物体中的新生长，如酵母、果蝇、蠕虫、小鼠和人类。从模式丝状真菌A. nidulans。正如预期的那样，A. nidulansseptins含有在其它septins中看到的高度保守的GTP结合和卷曲螺旋结构域。根据克隆与染色体特异性文库的杂交以及与A.在nidulanphysical map中，septins不是成簇的，而是分散在整个基因组中。在系统发育分析中，大多数真菌分隔蛋白可以与原型S.嗜热菌肽，Cdc 3，Cdc 10，Cdc 11和Cdc 12。内含子-外显子结构在septin类中是保守的。这项研究的结果表明，大多数真菌septins属于四个orthopathic类之一。
Members of the septin gene family are involved in cytokinesis and the organization of new growth in organisms as diverse as yeast, fruit fly, worm, mouse, and human. Five septin genes have been cloned and sequenced from the model filamentous fungusA. nidulans. As expected, theA. nidulansseptins contain the highly conserved GTP binding and coiled-coil domains seen in other septins. On the basis of hybridization of clones to a chromosome-specific library and correlation with anA. nidulansphysical map, the septins are not clustered but are scattered throughout the genome. In phylogenetic analysis most fungal septins could be grouped with one of the prototypicalS. cerevisiaeseptins, Cdc3, Cdc10, Cdc11, and Cdc12. Intron-exon structure was conserved within septin classes. The results of this study suggest that most fungal septins belong to one of four orthologous classes.