GROWTH CONE ADVANCE IS INVERSELY PROPORTIONAL TO RETROGRADE F-ACTIN FLOW
GROWTH CONE ADVANCE IS INVERSELY PROPORTIONAL TO RETROGRADE F-ACTIN FLOW
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DOI：
10.1016/0896-6273(95)90220-1
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发表时间：
1995-04-01
期刊：
影响因子：
16.2
通讯作者：
FORSCHER, P
中科院分区：
文献类型：
--
作者：
LIN, CH;FORSCHER, P
在先前的一项研究中，f -肌动蛋白似乎在引导微管在生长锥靶相互作用中起关键作用。在这里，我们评估了f -肌动蛋白流动模式，以研究f -肌动蛋白流动、微管/细胞器突出和生长速率之间的关系。我们首先证明了表面标记物（珠）以与底层f -肌动蛋白相同的速度移动的条件。然后用激光镊子定位这些珠子，以评估目标相互作用期间f -肌动蛋白的运动。我们发现逆行的f -肌动蛋白流动沿着目标相互作用轴衰减，与生长锥前进的速度成正比。与相互作用轴相邻的逆行肌动蛋白流动未受干扰。我们的研究结果表明，生长锥通过调节f -肌动蛋白-底物耦合效率，将逆行的f -肌动蛋白通量转化为正向运动。
In a previous study, F-actin appeared to play a key role in guiding microtubules during growth cone-target interactions. Here, F-actin flow patterns were assessed to investigate the relationship among F-actin flow, microtubde/organelle protrusion, and rates of outgrowth. We first demonstrated conditions in which surface markers (beads) moved at the same rate as underlying F-actin. These beads were then positioned, using laser tweezers, to assess F-actin movements during target interactions. We found retrograde F-actin flow was attenuated specifically along the target interaction axis in direct proportion to the rate of growth cone advance. Retrograde actin flow adjacent to the interaction axis was unperturbed. Our results suggest that growth cones transduce retrograde F-actin flux into forward movement by modulating F-actin-substrate coupling efficiency.