BLASTICIDIN S-RESISTANCE GENE (BSR) - A NOVEL SELECTABLE MARKER FOR MAMMALIAN-CELLS
BLASTICIDIN S-RESISTANCE GENE (BSR) - A NOVEL SELECTABLE MARKER FOR MAMMALIAN-CELLS
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DOI：
10.1016/0014-4827(91)90427-v
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发表时间：
1991-12-01
影响因子：
3.7
通讯作者：
HANAOKA, F
中科院分区：
文献类型：
--
作者：
IZUMI, M;MIYAZAWA, H;HANAOKA, F
Blasticidin S是一种抑制原核生物和真核生物蛋白质合成的微生物抗生素。将从蜡样芽孢杆菌k55 - s1株中分离得到的抗囊胚素s抗性基因（bsr）插入pSV2质粒载体，转染后导入培养的哺乳动物细胞。该基因被整合到基因组中，并赋予HeLa细胞对囊胚杀虫素S的抗性。bsr基因的转染频率与氨基糖苷磷酸转移酶基因一样高，即所谓的新基因，是哺乳动物细胞具有代表性的选择性标记。将多个bsrs拷贝整合到基因组中的转染物对囊胚杀虫素S具有高度抗性。此外，囊胚杀虫素S比G418更快地杀死细胞，G418通常被用作新基因的选择性药物。结果表明，红唇草可作为哺乳动物细胞的耐药性标记物。
Blasticidin S is a microbial antibiotic that inhibits protein synthesis in both prokaryotes and eukaryotes. The blasticidin S-resistance gene (bsr), isolated fromBacillus cereusK55-S1 strain, was inserted into pSV2 plasmid vector and introduced into cultured mammalian celte by transfection. Thebsrgene was integrated into the genome and conferred blasticidin S resistance on HeLa cells. The transfection frequency of thebsrgene was as high as that of the aminoglycoside phosphotransferase gene, the so-calledneogene, which is a representative selectable marker for mammalian cells. Transfectants in which several copies ofbsrhad been integrated into the genome were highly resistant to blasticidin S. Furthermore, blasticidin S killed the cells more rapidly than G418, which is conventionally used as a selective drug for theneogene. Thusbsris concluded to be useful as a drug-resistance marker for mammalian cells.