Role of Na+/H+ exchange in thrombin- and arachidonic acid-induced Ca2+ influx in platelets.

Role of Na+/H+ exchange in thrombin- and arachidonic acid-induced Ca2+ influx in platelets.
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Na /H 交换在凝血酶和花生四烯酸诱导的血小板 Ca2+ 流入中的作用。

DOI：
10.1016/0005-2736(88)90018-1
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发表时间：
1988
期刊：
Biochimica et biophysica acta
影响因子：
--
通讯作者：
A. Bosìa
A. Bosìa
中科院分区：
--
文献类型：
--
作者：
D. Ghigo;S. Treves;F. Turrini;A. Pannocchia;G. Pescarmona;A. Bosìa

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Platelet activation is accompanied by an increase of cytosolic free Ca2+concentration [Ca2+]i, (due to both extracellular Ca2+influx and Ca2+movements from the dense tubular system) and an Na+influx associated with H+extrusion. The latter event is attributable to the activation of Na+/H+exchange, which requires Na+in the extracellular medium and is inhibited by amiloride and its analogs. The present study was carried out to determine whether a link exists between Ca2+transients (measured by the quin2 method and the45CaCl2technique) and Na+/H+exchange activation (studied with the pH-sensitive intracellular probe, 6-carboxyfluorescein) during platelet stimulation. Washed human platelets, stimulated with thrombin and arachidonic acid, showed: (1) a large and rapid [Ca2+]irise, mostly due to a Ca2+influx through the plasma membrane; (2) a marked intracellular alkalinization. Both phenomena were markedly inhibited in the absence of extracellular Na+or in the pesence of an amiloride analog (EIPA). Monensin, a cation exchanger which elicits Na+influx and alkalinization, and NH4Cl, which induces alkanization only, were able to evoke an increase in [Ca2+]i, mostly as an influx from the extracellular medium. Our results suggest that Ca2+influx induced by thrombin and arachidonic acid in human platelets is strictly dependent on Na+/H+-exchange activation.
Inhibitors of Na+/H+ exchange block epinephrine- and ADP-induced stimulation of human platelet phospholipase C by blockade of arachidonic acid release at a prior step.
Na /H 交换抑制剂通过在先前步骤中阻断花生四烯酸释放来阻断肾上腺素和 ADP 诱导的人血小板磷脂酶 C 的刺激。
DOI： --
发表时间： 1986
期刊： The Journal of biological chemistry
影响因子： --
作者：
Sweatt,JD;Blair,IA;Cragoe,EJ;Limbird,LE
通讯作者： Limbird,LE
Evidence that Na+/H+ exchange regulates receptor-mediated phospholipase A2 activation in human platelets.
Na /H 交换调节人血小板中受体介导的磷脂酶 A2 激活的证据。
DOI： --
发表时间： 1986
期刊： The Journal of biological chemistry
影响因子： --
作者：
Sweatt,JD;Connolly,TM;Cragoe,EJ;Limbird,LE
通讯作者： Limbird,LE
Influence of sodium on the alpha 2-adrenergic receptor system of human platelets. Role for intraplatelet sodium in receptor binding.
钠对人血小板α2-肾上腺素能受体系统的影响。
DOI： --
发表时间： 1983
期刊： The Journal of biological chemistry
影响因子： --
作者：
Motulsky,HJ;Insel,PA
通讯作者： Insel,PA
Lipopolysaccharide and phorbol esters induce differentiation but have opposite effects on phosphatidylinositol turnover and Ca2+ mobilization in 70Z/3 pre-B lymphocytes.
脂多糖和佛波酯可诱导分化，但对 70Z/3 前 B 淋巴细胞中的磷脂酰肌醇周转和 Ca2+ 动员具有相反的作用。
DOI： --
发表时间： 1985
期刊： The Journal of biological chemistry
影响因子： --
作者：
Rosoff,PM;Cantley,LC
通讯作者： Cantley,LC