Genes that integrate multiple adipogenic signaling pathways in human mesenchymal stem cells
Genes that integrate multiple adipogenic signaling pathways in human mesenchymal stem cells
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DOI：
10.1016/j.bbrc.2011.05.089
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发表时间：
2011-06-17
影响因子：
3.1
通讯作者：
Aizawa, Yasunori
中科院分区：
文献类型：
--
作者：
Ito, Tomoya;Tsuruta, So;Aizawa, Yasunori
脂肪生成是一个充分表征的细胞分化过程。大量证据揭示了控制脂肪生成的核心转录因子和信号通路，但这些细胞信号之间的交互作用及其功能后果尚未得到彻底研究。因此，我们试图鉴定在人类间充质干细胞脂肪形成过程中受多种信号通路调控的基因。着眼于脂肪形成的早期阶段，微阵列分析和定量 RT-PCR 鉴定了 12 个基因，其转录水平受到完整脂肪形成诱导混合物的显着影响，但不受混合物的各个成分的影响。这些基因的表达动力学表明脂肪形成过程中转录调控的多种机制。这些基因与脂肪形成分化之间的功能关系常常是未知的。因此，这项研究提供了新的脂肪形成基因候选物，可能介导人类间充质干细胞内多个信号通路之间的通讯。 (C) 2011 Elsevier Inc. 保留所有权利。
Adipogenesis is a well-characterized cell differentiation process. A large body of evidence has revealed the core transcription factors and signaling pathways that govern adipogenesis, but cross-talks between these cellular signals and its functional consequences have not been thoroughly investigated. We, therefore, sought to identify genes that are regulated by multiple signaling pathways during adipogenesis of human mesenchymal stem cells. Focusing on the early stage of adipogenesis, microarray analysis and quantitative RT-PCR identified 12 genes whose transcription levels were dramatically affected by the complete adipogenic induction cocktail but not by the cocktail's individual components. Expression kinetics of these genes indicate diverse mechanisms of transcriptional regulation during adipogenesis. Functional relationships between these genes and adipogenic differentiation were frequently unknown. This study thus provided novel adipogenic gene candidates that likely mediate communications among multiple signaling pathways within human mesenchymal stem cells. (C) 2011 Elsevier Inc. All rights reserved.