A retinal-binding protein mediates olfactory attraction in the migratory locusts.
A retinal-binding protein mediates olfactory attraction in the migratory locusts.
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DOI：
10.1016/j.ibmb.2019.103214
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发表时间：
2019-08
影响因子：
3.8
通讯作者：
Zongyuan Ma;Jipeng Liu;Xiaojiao Guo
中科院分区：
文献类型：
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作者：
Zongyuan Ma;Jipeng Liu;Xiaojiao Guo
细胞视网膜色素脱结合蛋白（CRALBP）在视网膜中大量表达，其突变与视觉障碍有关。CRALBP在视网膜外组织中的功能鲜为人知。在此，我们研究了CRALBP在调节群居和独居蝗虫嗅觉行为中的功能。cralbpmRNA和蛋白质在蝗虫脑和触角中表达丰富。RNAi敲低cralbpin降低了群居蝗虫对群居挥发物的引诱反应。RNA-seq和定量PCR证实，章鱼胺受体α1（OctαR1）激活和抑制后，cralbpmRNA和蛋白表达水平分别上调和下调。基因网络分析表明，cralbp是激活和抑制OctαR1所产生的差异表达转录本（DET）相互作用网络的核心枢纽基因。此外，cralbpRNAi敲低抑制了章鱼胺（OA）-OctαR1信号对嗅觉吸引的诱导作用。CRALBP有助于传递OA信号，介导对群居挥发物中的两种气味成分愈创木酚和藜芦醇的嗅觉吸引反应。提示CRALBP可能是OctαR1信号通路中的一个新的效应蛋白，参与嗅觉吸引的调控。这项研究表明，CRALBP调节嗅觉吸引在视网膜外组织和视黄醇代谢可能是至关重要的嗅觉吸引调制。
Cellular retinaldehyde-binding protein (CRALBP) is abundantly expressed in retina and its mutations are associated with visual impairments. The functions of CRALBP are less known in extra retinal tissues. Herein, we study the function of CRALBP in modulating olfactory behaviors in gregarious and solitary locusts. The expressions ofcralbpmRNA and protein were enriched in locust brains and antennae. RNAi knockdown ofcralbpin gregarious locusts decreased their attractive response to gregarious volatiles. RNA-seq and quantitative PCR confirmed thatcralbpmRNA and protein expression levels were upregulated and downregulated after octopamine receptor α1 (OctαR1) activation and inhibition, respectively. Gene network analysis revealed thatcralbpis the core hub gene in the interactive network among differentially expressed transcripts (DETs) resulting from activating and inhibiting OctαR1. Moreover,cralbpRNAi knockdown inhibited the induction of olfactory attraction by octopamine (OA)-OctαR1 signaling. CRALBP helped to transmit OA signals to mediate olfactory attraction response to guaiacol and veratrole, which are two odorant components in gregarious volatiles. This study suggested that CRALBP may act as a novel effector protein in OctαR1 signaling to mediate olfactory attraction. This study indicated that CRALBP modulates olfactory attraction in extra retina tissues and retinaldehyde metabolism may be crucial for olfactory attraction modulation.