GENETIC-ANALYSIS OF TEMPERATURE-SENSITIVE EMBRYOGENESIS MUTANTS IN CAENORHABDITIS-ELEGANS
GENETIC-ANALYSIS OF TEMPERATURE-SENSITIVE EMBRYOGENESIS MUTANTS IN CAENORHABDITIS-ELEGANS
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DOI：
10.1016/0012-1606(81)90383-3
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发表时间：
1981-01-01
影响因子：
2.7
通讯作者：
VONEHRENSTEIN, G
中科院分区：
文献类型：
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作者：
CASSADA, R;ISNENGHI, E;VONEHRENSTEIN, G
线虫C中存在大量温度敏感（ts）胚胎停滞突变体。elegans被分离出来。在甲基磺酸乙酯诱变后，通过16 - 25 ℃的复制平板接种从10，000个分离的克隆中鉴定ts突变体。C.当适当的发育阶段上移时，至少有54个独立的ts发育突变体表现出胚胎停滞。这些代表了来自155只诱变动物的294个ts克隆的28%的独立发育突变体。初步表征这一子集的emb突变体的报告。大多数在胚胎发育的中、晚期停止，并伴有异常的终末表型。额外的非胚胎停滞阶段（取决于上移方案）表明，许多emb基因也需要前或胚后发育。对于大多数胚胎突变体而言，亲本基因表达足以在25 ℃下进行胚胎发育。C.通过对37个新的emb突变体和32个已分离的emb突变体的互补性分析，获得了25个新的emb基因。这使得C中定义的胚胎停滞基因总数。54.第54章新的基因被大致定位。在本集中，10 emb基因图中的中间连锁群III。第二等位基因的出现频率给出了胚胎发生所需的基因数量约为200的1个估计值（通过泊松分析）。第二个估计是从筛选的所有ts致死突变体中28%的emb突变体的分数获得的，对于C.这对应于胚胎发生所需的560个基因。高度可变基因的一个小子集也被描述。本筛选emb突变体的结果进行了比较，与其他人。几个ts突变体给出了复杂的连锁结果（显然不是由于双突变），表明染色体重排。
A large set of temperature-sensitive (ts) embryonic arrest mutants in the nematode C. elegans was isolated. Following ethylmethane sulfonate mutagenesis, ts mutants were identified from 10,000 segregated clones by replica plating from 16-25.degree. C. A least 54 independent ts developmental mutants showed embryonic arrest when the appropriate developmental stage was shifted up. These represent 28% of the independent developmental mutants from 294 ts clones from 155 mutagenized animals. Initial characterization of this subset of emb mutants is reported. Most of them arrest in middle or late embryogenesis with abnormal terminal phenotypes. Additional nonembryonic arrest stages (depending on shift-up regimen) suggest that many emb genes also are required for pre- or postembryonic development. For most of the emb mutnats parental gene expression is sufficient for embryonic development at 25.degree. C. Based on complementation analysis of 37 new emb mutants together with 32 isolated by other investigators, 25 new emb genes are reported. This brings the total of embryonic arrest genes defined in C. elegans to 54. The new genes were mapped approximately. In the present set, 10 emb genes map in the middle of linkage group III. The frequency of occurrence of 2nd alleles gives 1 estimate (by Poisson analysis) for the number of genes required for embryogenesis of about 200. A 2nd estimate is obtained from the fraction of 28% emb mutants among all ts lethal mutants in the screen; for an estimated 2000 essential genes in C. elegans this corresponds to 560 genes required for embryogenesis. A small subset of highly mutable genes also is described. Results of the present screen for emb mutants are compared to those of others. Several ts mutants gave complex linkage results (apparently not due to double mutations), suggesting chromosome rearrangements.