End of the Semester Review of Biochemistry Using a Case Study on Phosphoglucose Isomerase Deficiency
End of the Semester Review of Biochemistry Using a Case Study on Phosphoglucose Isomerase Deficiency
复制标题
生物化学学期末复习以磷酸葡萄糖异构酶缺乏症为例
DOI：
10.1021/acs.jchemed.0c00540
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发表时间：
2020
影响因子：
3
通讯作者：
A. Nag
中科院分区：
文献类型：
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作者：
A. Nag
案例研究练习被广泛用作教学工具，不仅可以传递内容，还可以让学生利用现实生活场景解决问题。由于 COVID-19，向虚拟学习平台的过渡使得实施探究驱动的学习练习变得更加必要，该练习将各种生物化学概念捆绑到一项活动中。在这里，我提出了一个基于案例的活动，用于回顾和整合生物化学入门课程中教授的多个概念。本案例研究源自一名新生儿的临床数据，该新生儿被诊断患有与磷酸葡萄糖异构酶缺陷有关的溶血性贫血，以及实验室生成的比较野生型和突变蛋白的研究数据。这个数据驱动的案例研究采用问题引导的方法来回顾酸碱化学、蛋白质结构、蛋白质纯化、蛋白质表征、酶动力学、机制、核酸化学、碳水化合物化学和代谢。这项活动分两个讲座时段进行，以便有足够的时间运用整个学期学到的概念来解决本活动中的问题。此外，案例研究帮助学生识别一些具有挑战性的概念，并指导他们为累积期末考试生成学习清单。该活动在面对面的课堂环境和使用 Blackboard Collaborate Ultra 的同步虚拟教室中进行。此次活动的调查清楚地表明了学生对两种模式参与案例学习的热情。
Case study exercises are extensively used as a pedagogical tool not only to deliver the content but also to engage students in problem-solving using real-life scenarios. The transition to the virtual learning platform due to COVID-19 made it even more imperative to implement an inquiry-driven learning exercise that tethers various biochemistry concepts into one activity. Here, I present a case-based activity that was used to review and integrates multiple concepts taught in the introductory biochemistry course. This case study is derived from the clinical data of a newborn, diagnosed with hemolytic anemia pertaining to a defect in the phosphoglucose isomerase enzyme, and laboratory-generated research data comparing the wild type and the mutant proteins. This data-driven case study takes a question-guided approach to review acid–base chemistry, protein structure, protein purification, protein characterization, enzyme kinetics, mechanism, nucleic acids chemistry, carbohydrate chemistry, and metabolism. This activity is conducted over two lecture periods, allowing enough time to implement concepts learned during the entire semester to solve the questions in this activity. Furthermore, the case study helps students to identify some of the challenging concepts and guides them to generate a study list for the cumulative final exam. This activity has been conducted both in a face-to-face classroom setting and in a synchronous virtual classroom using Blackboard Collaborate Ultra. The survey of this activity clearly shows students’ enthusiasm toward participating in case-based learning in both modalities.