Decreased intestinal nutrient response in diet-induced obese rats: role of gut peptides and nutrient receptors
Decreased intestinal nutrient response in diet-induced obese rats: role of gut peptides and nutrient receptors
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DOI：
10.1038/ijo.2012.45
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发表时间：
2013-03-01
影响因子：
4.9
通讯作者：
Covasa, M.
中科院分区：
文献类型：
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作者：
Duca, F. A.;Swartz, T. D.;Covasa, M.
背景和目标：饮食诱导的肥胖症（DIO）是一种用于检测人类肥胖症的极好模型，其包括基因型和环境（饮食）因素。对外周饱腹感信号的反应性降低可能是该模型中摄食过多的原因。在这项研究中，我们研究了喂养高能量/高脂肪（HE/HF）饮食的远交DIO和DIO抵抗（DR）大鼠对营养诱导饱腹的反应，以及肠道饱腹肽含量，肠道营养反应受体丰度和迷走神经厌食受体表达。在营养物（葡萄糖和胰岛素（IL））胃负荷后，测试喂食HE/HF饮食的远系DIO和DR大鼠的短期进食反应。检测DIO和DR动物近端肠上皮细胞肠饱肽和脂肪酸反应性受体的基因和蛋白表达以及结状神经节胆囊收缩素1受体（CCK-1 R）和瘦素受体（LepR）mRNA的表达。结果：DIO大鼠对IL-（P
BACKGROUND AND AIMS: Diet-induced obesity (DIO) is an excellent model for examining human obesity comprising both genotypic and environmental (diet) factors. Decreased responsiveness to peripheral satiety signaling may be responsible for the hyperphagia in this model. In this study, we investigated responses to nutrient-induced satiation in outbred DIO and DIO-resistant (DR) rats fed a high-energy/high-fat (HE/HF) diet as well as intestinal satiety peptide content, intestinal nutrient-responsive receptor abundance and vagal anorectic receptor expression.METHODS: Outbred DIO and DR rats fed a HE/HF diet were tested for short-term feeding responses following nutrient (glucose and intralipid (IL)) gastric loads. Gene and protein expressions of intestinal satiety peptides and fatty acid-responsive receptors were examined from isolated proximal intestinal epithelial cells and cholecystokinin-1 receptor (CCK-1R) and leptin receptor (LepR) mRNA from the nodose ganglia of DIO and DR animals.RESULTS: DIO rats were less responsive to IL-(P