BIOCHEMICAL EVIDENCE PERTAINING TO THE TAXONOMIC RELATIONSHIPS WITHIN THE FAMILY CHAMAELEONIDAE

BIOCHEMICAL EVIDENCE PERTAINING TO THE TAXONOMIC RELATIONSHIPS WITHIN THE FAMILY CHAMAELEONIDAE
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DOI:
10.1163/156853891x00428
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发表时间:
1991-01-01
期刊:
影响因子:
1.6
通讯作者:
ARNTZEN J W
ARNTZEN J W
中科院分区:
生物学3区
文献类型:
--
作者:
HOFMAN A;MAXSON L R;ARNTZEN J W

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采用两种不同的生化方法研究了变色龙科代表性物种之间的关系:在 18-21 个假定位点进行淀粉凝胶电泳,以建立相对较近的亲缘物种的关系,并使用微补体固定(MC'F)的定量免疫学技术来探测更远的系统发育关系。用非洲变色龙和马达加斯加变色龙的血清白蛋白制备抗血清。我们的结果与基于其他性状的系统发育假设进行了比较(Klaver,1981a;Klaver 和 Bohme,1986)。这项研究最重要的结论是:1) Chamaeleonidae 形成一个古老的类群,其历史可以追溯到白垩纪,2) Mertens (1966) 认为 Chamaeleo 属是并系群。将非洲和马达加斯加可能分离时间的地质估计与“白蛋白分子钟”的估计进行比较。
Relationships among representative species of the family Chamaeleonidae were investigated using two different biochemical approaches: starch gel electrophoresis at 18-21 presumptive loci was used to establish the relationships of relatively closely allied species and the quantitative immunological technique of microcomplement fixation (MC'F) was used to probe more distant phylogenetic relationships. Antisera were made to serum albumin of the African Chamaeleo quilensis and the Madagascan Chamaeleo pardalis. Our results are compared to phylogenetic hypotheses based on other characters (Klaver, 1981a; Klaver and Bohme, 1986). The most important conclusions of this study are that 1) the Chamaeleonidae form an old group, dating back into the Cretaceous, and 2) the genus Chamaeleo as recognized by Mertens (1966) is paraphyletic. Geological estimates of the probable time of separation of Africa and Madagascar are compared to estimates from the "albumin molecular clock".