In vitro enhancement of the mutagenicity of 4-nitro-o-phenylenediamine by plant S-9.
In vitro enhancement of the mutagenicity of 4-nitro-o-phenylenediamine by plant S-9.
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植物 S-9 体外增强 4-硝基-邻苯二胺的致突变性。
DOI：
10.1002/em.2860070104
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Plewa,MJ
中科院分区：
文献类型：
--
作者：
Gentile,JM;Gentile,GJ;Townsend,S;Plewa,MJ
直接作用诱变剂4-硝基邻苯二胺（NOP）在体外被豌豆或烟草S-9激活，在鼠伤寒沙门氏菌菌株TA 98中成为更强效的诱变剂。Aroclor 1254诱导的大鼠肝脏S-9未改变NOP致突变性。植物S-9对NOP的活化是热敏的，但不依赖于NADPH，不涉及超氧自由基，也不受CO的抑制。植物过氧化物酶与NOP活化之间建立了直接关系。几种纯化的过氧化物酶，包括辣根过氧化物酶，氯过氧化物酶和乳过氧化物酶也激活NOP。过氧化过程不是H2 O2依赖性的，但被过氧化物酶抑制剂部分抑制。
The direct‐acting mutagen 4‐nitro‐o‐phenylenediamine (NOP) was activated in vitro by pea or tobacco S‐9 into a more potent mutagen inSalmonella typhimuriumstrain TA98. NOP mutagenicity was not altered by Aroclor 1254‐induced rat liver S‐9. The plant S‐9 activation of NOP was heat‐sensitive but was not NADPH‐dependent, did not involve superoxide radicals, and was not inhibited by CO. A direct relationship between plant peroxidase and NOP activation was established. Several purified peroxidases including horseradish peroxidase, chloroperoxidase, and lactoperoxidase also activated NOP. The perodoxidative process was not H2O2‐dependent but was partially inhibited by a peroxidase inhibitor.