Microwave-assisted extraction and in situ clean-up for the determination of fluoroquinolone antibiotics in chicken breast muscle by LC-MS/MS.
Microwave-assisted extraction and in situ clean-up for the determination of fluoroquinolone antibiotics in chicken breast muscle by LC-MS/MS.
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DOI：
10.1002/jssc.201000365
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发表时间：
2011
影响因子：
3.1
通讯作者：
Haoyan Xu;Ligang Chen;Lei Sun;Xin Sun;Xiaobo Du;Jian Wang;Tianyi Wang;Qing-Xiao Zeng;Hui Wang;Yang Xu;Xiaopan Zhang;L. Ding
中科院分区：
文献类型：
--
作者：
Haoyan Xu;Ligang Chen;Lei Sun;Xin Sun;Xiaobo Du;Jian Wang;Tianyi Wang;Qing-Xiao Zeng;Hui Wang;Yang Xu;Xiaopan Zhang;L. Ding
建立了测定鸡胸肌中氟沙星、左氧氟沙星、环丙沙星、隆氟沙星、恩诺沙星、斯帕沙星6种氟喹诺酮类抗生素含量的新方法，采用微波辐照一步提取和净化。以ACN为0.3% v/v磷酸/水pH 3 （70:30, v/v）的混合物为萃取液，以己烷为净化液。采用液相色谱-串联质谱联用系统对提取物进行分析。日内rsd为1.0 ~ 10.4%，日内rsd为3.8% ~ 13.6%。在鸡胸肌3个强化水平（20、100和500 ng/g）下，氟喹诺酮类抗生素的回收率为66.0 ~ 97.2%。lod范围为2.7 ~ 6.7 ng/g。该方法简化了样品制备过程，减少了操作误差。
A new method was developed for the determination of six fluoroquinolone antibiotics including fleroxacin, levofloxacin, ciprofloxacin, lomfloxacin, enrofloxacin, and sparfloxacin in chicken breast muscle, in which the extraction and clean-up were performed in one step by microwave irradiation. The mixture of ACN containing 0.3% v/v phosphoric acid/water pH 3 (70:30, v/v) was used as the extraction solution and hexane was used as the clean-up solution. The extract was analyzed by liquid chromatography-tandem mass spectrometry system. The RSDs of intra- and inter-day obtained are in the range of 1.0-10.4 and 3.8-13.6%, respectively. In the three fortified levels of chicken breast muscle (20, 100, and 500 ng/g), the recoveries of fluoroquinolone antibiotics ranging from 66.0 to 97.2% are obtained. The LODs are in the range of 2.7-6.7 ng/g. This method simplifies the process of the sample preparation and reduces the operation errors.