Exploration of miRNA and mRNA Profiles in Fresh and Frozen-Thawed Boar Sperm by Transcriptome and Small RNA Sequencing
Exploration of miRNA and mRNA Profiles in Fresh and Frozen-Thawed Boar Sperm by Transcriptome and Small RNA Sequencing
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通过转录组和小 RNA 测序探索新鲜和冻融公猪精子中的 miRNA 和 mRNA 谱。
DOI：
10.3390/ijms20040802
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发表时间：
2019-02-02
影响因子：
5.6
通讯作者：
Zeng, Chang-Jun
中科院分区：
文献类型：
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作者：
Dai, Ding-Hui;Qazi, Izhar Hyder;Zeng, Chang-Jun
由于冷冻精液的产仔率较低，产仔数减少，90%以上的人工授精（AI）使用液体储存的公猪精液进行。尽管猪精子冷冻保存方案的优化已经取得了实质性进展，但与猪精子冷冻损伤和冷冻耐受性相关的影响因素和潜在机制仍然很大程度上未知。在这项研究中，我们报告的差异表达的mRNA和miRNA的新鲜和冻融公猪精子使用高通量RNA测序。结果表明，在新鲜和冻融的猪精子中，有567个mRNA和135个miRNA存在差异表达。基因本体论（GO）和京都基因和基因组百科全书（KEGG）富集分析显示，大多数DE mRNA在环境信息处理中富集，例如，丝氨酸-细胞因子受体相互作用、PI 3 K-Akt信号传导、细胞粘附、MAPK和钙信号传导途径。此外，DE miRNAs的靶点在重要的GO术语中富集，例如细胞过程、蛋白结合和对刺激的响应。总之，我们推测DE mRNA和miRNA在很大程度上参与了公猪精子对环境刺激的反应、凋亡和代谢活动。表达的差异也反映了精子在冷冻保存过程中的各种结构和功能变化。
Due to lower farrowing rate and reduced litter size with frozen-thawed semen, over 90% of artificial insemination (AI) is conducted using liquid stored boar semen. Although substantial progress has been made towards optimizing the cryopreservation protocols for boar sperm, the influencing factors and underlying mechanisms related to cryoinjury and freeze tolerance of boar sperm remain largely unknown. In this study, we report the differential expression of mRNAs and miRNAs between fresh and frozen-thawed boar sperm using high-throughput RNA sequencing. Our results showed that 567 mRNAs and 135 miRNAs were differentially expressed (DE) in fresh and frozen-thawed boar sperm. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that the majority of DE mRNAs were enriched in environmental information processing such as cytokine-cytokine receptor interactions, PI3K-Akt signaling, cell adhesion, MAPK, and calcium signaling pathways. Moreover, the targets of DE miRNAs were enriched in significant GO terms such as cell process, protein binding, and response to stimuli. In conclusion, we speculate that DE mRNAs and miRNAs are heavily involved in boar sperm response to environment stimuli, apoptosis, and metabolic activities. The differences in expression also reflect the various structural and functional changes in sperm during cryopreservation.