Ion-chelation based digital barcodes for multiplexing of a suspension array
Ion-chelation based digital barcodes for multiplexing of a suspension array
复制标题
用于悬浮阵列多重化的基于离子螯合的数字条形码
作者：
Feng Guangxia;Guan Tian;He Qinghua;Lu Bangrong;Chen Xuejing;Wang Bei;Zhou Xuesi;Ding Jiangfeng;He Yonghong
Ion-chelated microbeads (ICMs) for suspension arrays can be prepared by chelating metal ions (MIs), which are used as encoding materials. Stimulating the ICMs, laser induced breakdown spectra (LIBs) can be obtained and the atomic spectra of the chelated ions are chosen as the decoding signals. Our ICMs show digital characteristics with high stability due to the properties of LIBs. And, since there are many available coding materials and different kinds of coding materials can be easily combined, the coding capacity can be considerably enlarged. Further, the background interference in fluoroimmunoassay detection could be avoided, because the ICMs contain no fluorescence emission. In our studies, we achieved a total of 15 types of barcodes by taking full advantage of 4 kinds of ions, then a fluoroimmunoassay was performed to demonstrate the specificity and detection performance of our ICMs in multiplexing and the detection limit could reach 1.49 × 10−10 M, showing promising potential in applications.
