Label-free biochemical imaging of heart tissue with high-speed spontaneous Raman microscopy
Label-free biochemical imaging of heart tissue with high-speed spontaneous Raman microscopy
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DOI：
10.1016/j.bbrc.2009.03.028
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发表时间：
2009-05-01
影响因子：
3.1
通讯作者：
Takamatsu, Tetsuro
中科院分区：
文献类型：
--
作者：
Ogawa, Mitsugu;Harada, Yoshinori;Takamatsu, Tetsuro
无标记成像是阐明在体心脏组织的形态学和生化变化的理想方法。自发拉曼显微术（SRM）提供高化学对比度而无需标记。但是由于低灵敏度和随之而来的长成像时间而在获取图像方面存在缺点。在这里，我们报告了一种新的技术，大鼠心脏组织的无标记成像与高速SRM结合共振拉曼效应的血红素蛋白。我们发现，个别心肌细胞的共振拉曼信号产生的主要是减少15-和C-型细胞色素，心肌细胞和血管成像区分细胞色素从氧合和脱氧血红蛋白在完整的心脏，而心肌细胞和纤维化组织成像区分细胞色素从I型胶原在梗死心脏与主成分分析。这些结果表明SRM作为一种无标记的高对比度成像技术的潜力，提供了一种新的方法来研究生化变化，基于分子组成，在心脏。(C)2009 Elsevier Inc. All rights reserved.
Label-free imaging is desirable for elucidating morphological and biochemical changes of heart tissue in vivo. Spontaneous Raman microscopy (SRM) provides high chemical contrast without labeling. but presents disadvantage in acquiring images due to low sensitivity and consequent long imaging time. Here, we report a novel technique for label-free imaging of rat heart tissues with high-speed SRM combined with resonance Raman effect of heme proteins. We found that individual cardiomyocytes were identified with resonance Raman signal arising mainly from reduced 15- and c-type cytochromes, and that cardiomyocytes and blood vessels were imaged by distinguishing cytochromes from oxy- and deoxyhemoglobin in intact hearts, while cardiomyocytes and fibrotic tissue were imaged by distinguishing cytochromes from collagen type-I in infarct hearts with principal component analysis. These results suggest the potential of SRM as a label-free high-contrast imaging technique, providing a new approach for studying biochemical changes, based on the molecular composition, in the heart. (C) 2009 Elsevier Inc. All rights reserved.