Novel electrochemical sensor system for protein using the aptamers in sandwich manner
Novel electrochemical sensor system for protein using the aptamers in sandwich manner
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DOI：
10.1016/j.bios.2004.09.002
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发表时间：
2005-04-15
影响因子：
12.6
通讯作者：
Sode, K
中科院分区：
文献类型：
--
作者：
Ikebukuro, K;Kiyohara, C;Sode, K
利用适体构建了一种新型的夹心式蛋白质电化学检测系统。选择识别凝血酶不同位置的两种不同适配子构建夹心式蛋白质传感系统，其中一种固定在金电极上，用于将凝血酶捕捉到电极上，另一种用于检测。为了获得信号，用吡咯喹啉醌葡萄糖脱氢酶((PQQ)GDH)标记被检测的适配子，并测量凝血酶、金固定的适配子和(PQQ)GDH标记的适配子在电极上形成络合物后葡萄糖加成产生的电流。(PQQ)GDH产生的电流随凝血酶浓度的增加而增加，并选择性地检测到10 nM以上的凝血酶。将两种不同的适配子夹在凝血酶中，构建了间歇式蛋白质传感系统，在40-100 nm范围内与凝血酶浓度的增加呈线性关系。(C)2004爱思唯尔B.V.保留所有权利。
Novel electrochemical detection system for protein in sandwich manner using the aptamers was developed. Two different aptamers, which recognize different positions of thrombin, were chosen to construct sandwich type sensing system for protein, and one was immobilized onto the gold electrode for capturing thrombin onto the electrode and the other was used for detection. To obtain the signal, the aptamer for detection was labeled with pyrroquinoline quinone glucose dehydrogenase ((PQQ)GDH), and the electrical current, generated from glucose addition after the formation of the complex of thrombin, gold immobilized aptamer and the (PQQ)GDH labeled aptamer on the electrode, was measured. The increase of the electric current generated by (PQQ)GDH was observed in dependent manner of the concentration of thrombin added, and more than 10 nM thrombin was detected selectively. The batch type protein sensing system was constructed using the two different aptamers sandwiching thrombin and it showed linear response to the increase of the thrombin concentration in the range of 40-100 nM. (c) 2004 Elsevier B.V. All rights reserved.