RNA Sequencing Analysis of Gene Expression by Electroacupuncture in Guinea Pig Gallstone Models.
RNA Sequencing Analysis of Gene Expression by Electroacupuncture in Guinea Pig Gallstone Models.
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电针对豚鼠胆结石模型基因表达的RNA测序分析
DOI：
10.1155/2022/3793946
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Li Z
中科院分区：
文献类型：
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作者：
Hao M;Dou Z;Xu L;Shao Z;Sun H;Li Z
背景临床研究表明，电针（EA）可促进胆囊蠕动并缓解胆结石。然而，EA 对胆结石影响的机制尚不清楚。本研究利用mRNA转录组分析来研究EA可能的治疗靶点。方法采用Hartley SPF豚鼠建立胆结石模型。采用Illumina NovaSeq 6000平台对正常组（Normal）、胆结石模型组（Model）和电针治疗组（EA）豚鼠胆囊进行RNA测序。在模型与正常以及 EA 与模型中分别检查不同表达的基因 (DEG)。通过比较模型与正常和 EA 与模型的 DEG，选择 EA 逆转的 DEG。通过基因本体（GO）分析丰富了生物学功能。分析了蛋白质-蛋白质相互作用（PPI）网络。结果 EA 2 周后，模型与正常相比有 257 个 DEG，EA 与模型有 1704 个 DEG。在这些DEG中鉴定出94个被EA逆转的DEG，其中包括28个逆转的上调DEG和66个逆转的下调DEG。通过PPI网络分析，Cytoscape的Cytohubba插件发现了10个hub基因。实时定量 PCR (qRT-PCR) 验证了这些变化。结论 我们鉴定了一些可能在胆结石治疗中发挥关键作用的 GO 和基因。这项研究可能有助于了解电针治疗胆结石的机制。
Background Clinical studies have shown that electroacupuncture (EA) promotes gallbladder motility and alleviates gallstone. However, the mechanism underlying the effects of EA on gallstone is poorly understood. In this study, the mRNA transcriptome analysis was used to study the possible therapeutic targets of EA. Methods Hartley SPF guinea pigs were employed for the gallstone models. Illumina NovaSeq 6000 platform was used for the RNA sequencing of guinea pig gallbladders in the normal group (Normal), gallstone model group (Model), and EA-treated group (EA). Differently expressed genes (DEGs) were examined separately in Model vs. Normal and EA vs. Model. DEGs reversed by EA were selected by comparing the DEGs of Model vs. Normal and EA vs. Model. Biological functions were enriched by gene ontology (GO) analysis. The protein-protein interaction (PPI) network was analyzed. Results After 2 weeks of EA, 257 DEGs in Model vs. Normal and 1704 DEGs in EA vs. Model were identified. 94 DEGs reversed by EA were identified among these DEGs, including 28 reversed upregulated DEGs and 66 reversed downregulated DEGs. By PPI network analysis, 10 hub genes were found by Cytohubba plugin of Cytoscape. Quantitative real-time PCR (qRT-PCR) verified the changes. Conclusion We identified a few GOs and genes that might play key roles in the treatment of gallstone. This study may help understand the therapeutic mechanism of EA for gallstone.