Clinical usefulness of serum EBV DNA levels of BamHI W and LMP1 for nasal NK/T-cell lymphoma
Clinical usefulness of serum EBV DNA levels of BamHI W and LMP1 for nasal NK/T-cell lymphoma
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DOI：
10.1002/jmv.20853
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发表时间：
2007-05-01
影响因子：
12.7
通讯作者：
Harabuchi, Yasuaki
中科院分区：
文献类型：
--
作者：
Ishii, Hideyuki;Ogino, Takeshi;Harabuchi, Yasuaki
采用实时定量聚合酶链反应（PCR）检测20例鼻腔自然杀伤细胞(NK)/ t细胞淋巴瘤患者血清BamHI W片段和潜伏膜蛋白1 (LMP1) EBV DNA水平。两种血清EBV dna在所有患者中均检测到高水平，但在所有健康对照中均低于检测极限。在10例（50%）患者的淋巴瘤细胞中检测到BamHI Z片段，Epstein-Barr-replication activator （ZEBRA）表达。表达ZEBRA的患者BamHI W和LMP1的DNA水平显著升高（P=0.0081， P=0.004），提示EBV DNA可能是由EBV从淋巴瘤细胞复制引起的。Kaplan-Meier和单变量分析显示，治疗前高水平的BamHI W和LMP1 DNA水平以及治疗后高水平的BamHI W DNA水平与较短的无病生存期和总生存期相关（P < 0.05）。尽管BamHI W和LMP1的DNA水平显著相关，但它们的动态并不总是平行的。治疗前两种EBV dna水平较低的患者病程较好，而治疗前两种EBV dna水平较高的患者病程较差（P=0.0085）。更重要的是，这两种EBV dna的高预处理水平被确定为各种预后因素中唯一独立的预后因素。这些数据表明，同时测量血清中BamHI W和LMP1 dna的水平可能有助于鼻部NK/ t细胞淋巴瘤患者的诊断、疾病监测和预后预测。
Quantitative real-time polymerase chain reaction (PCR) was utilized to measure serum EBV DNA levels of BamHI W fragment and latent membrane protein 1 (LMP1) in 20 nasal natural killer (NK)/T-cell lymphoma patients. Both serum EBV DNAs were detected at high levels in all patients, but the levels were below the limit of detection in all healthy controls. The BamHI Z fragment, Epstein-Barr-replication activator (ZEBRA) expression was detected in a small proportion (0.1-3%) of lymphoma cells from 10 (50%) of the patients. Patients with ZEBRA expression showed significantly higher DNA levels of BamHI W and LMP1 (P=0.0081, P=0.004), suggesting that EBV DNA may be caused by EBV replication from lymphoma cells. Kaplan-Meier and univariate analyses revealed that high DNA levels of BamHI W and LMP1 at pre-treatment and high BamHI W DNA level at post-treatment were associated with short disease-free survival and overall survival (P < 0.05, each). Although the DNA levels of BamHI W and LMP1 correlated significantly, their dynamics were not always parallel. Patients with low pre-treatment level of both EBV DNAs showed a favorable course, in contrast to patients with high pre-treatment level of both EBV DNAs who showed an aggressive course (P=0.0085). More importantly, the high pre-treatment level of both EBV DNAs was determined as the only independent prognostic factor among various prognostic factors. These data suggest that simultaneous measurement of serum levels of both BamHI W and LMP1 DNAs may be useful for diagnosis, disease monitoring, and prediction of prognosis for nasal NK/T-cell lymphoma patients.