The effects of enamel matrix derivative on the proliferation and differentiation of human mesenchymal stem cells
The effects of enamel matrix derivative on the proliferation and differentiation of human mesenchymal stem cells
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DOI：
10.1111/j.1600-0501.2009.01901.x
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发表时间：
2010-07-01
影响因子：
4.3
通讯作者：
Lee, Baeksoo
中科院分区：
文献类型：
--
作者：
Jue, Seong-Suk;Lee, Won Young;Lee, Baeksoo
目的探讨釉质衍生物基质（elaminarderivativemartrix，EMD）对人骨髓间充质干细胞（humanmesenchymalstemcells，hMSCs）增殖、矿化和分化的影响。为了评价矿化，在成骨培养基中培养21天后进行茜素红S（ARS）试验。为了研究一些与骨相关的蛋白质，即I型胶原蛋白，（Col IA 2）、骨唾液酸蛋白（BSP）和骨γ-羧基谷氨酸（Gla）蛋白（BGly，骨钙素），在培养2、3和4周后进行实时聚合酶链反应（RT-PCR）测试，结果随着EMD浓度的增加，细胞增殖活性和矿化活性均显著增加（P < 0.05）。在对照组中，Col IA 2的表达降低，但EMD随时间的推移增强其表达，并与浓度相关。BSP的表达量随时间的推移而增加，但EMD在第四周显著抑制其表达。同样，在对照组中，随着培养时间的延长，BGly的表达量增加。结论在本研究范围内，EMD可促进hMSCs的增殖。ARS染色结果显示，EMD对成骨细胞矿化有促进作用; RT-PCR结果显示，EMD通过促进Col IA 2表达促进成骨细胞早期分化，但通过降低BSP和BG 3基因表达抑制矿化。EMD形成的矿化结节可能由正常骨以外的物质组成。由于骨的大部分有机基质是作为矿化位点的I型胶原，因此尽管非胶原蛋白的组分不同，骨或骨样矿化物质可能已经形成。引用这篇文章：Jue S-S，Lee WY，Kwon Y-D，Kim Y-R，Pae A，Lee B.釉基质衍生物对人骨髓间充质干细胞增殖和分化的影响。Res. 21，2010; 741-746.doi：10.1111/j.1600-0501.2009.01901.x.
PurposeThis study was designed to investigate the effect of enamel derivative matrix (EMD) on the proliferation, mineralization, and differentiation of human mesenchymal stem cells (hMSCs).Material and methodsFor the proliferation assay, water-soluble tetrazolium salt-8 tests were carried out after culturing for 24 and 48 h. For the evaluation of mineralization, Alizarin red S (ARS) tests were performed after 21 days of culturing in an osteogenic medium. In order to investigate some of the bone-related proteins, namely type I collagen (Col I A2), bone sialoprotein (BSP), and bone gamma-carboxyglutamate (Gla) protein (BGLAP, osteocalcin), real-time polymerase chain reaction (RT-PCR) tests were carried out after 2, 3, and 4 weeks of culturing, respectively.ResultsThe activity of proliferation and mineralization increased significantly depending on the concentration of EMD (P < 0.05). In the control group, the expression of Col I A2 decreased, but EMD enhanced its expression over time and was correlated to the concentration. The amount of expression of BSP in this group increased over time, but EMD strikingly suppressed its expression in the fourth week. As well, the amount of expression of BGLAP increased as the culture duration lengthened in the control group. However, the expression of BGLAP was suppressed in the experimental group with EMD.ConclusionWithin the limits of this study, EMD enhanced the proliferation of hMSCs. After evaluation with ARS staining, EMD seemed to enhance mineralization, and the RT-PCR test revealed that EMD promoted early-stage osteoblast differentiation by enhancing Col I A2 expression, but exerted an inhibitory effect on the mineralization by lowering the gene expression of BSP and BGLAP. Mineralized nodules formed with EMD may be composed of substances other than normal bone. Because most of the organic matrix of bone is type I collagen, which acts as the mineralization site, bone or bone-like mineralized mass might have been formed in spite of the different components of the non-collagenous proteins.To cite this article:Jue S-S, Lee WY, Kwon Y-D, Kim Y-R, Pae A, Lee B. The effects of enamel matrix derivative on the proliferation and differentiation of human mesenchymal stem cells.Clin. Oral Impl. Res. 21, 2010; 741-746.doi: 10.1111/j.1600-0501.2009.01901.x.