Comparison of graphical analyses for maize genetic experiments: Application of biplots and polar plot to line x tester design
Comparison of graphical analyses for maize genetic experiments: Application of biplots and polar plot to line x tester design
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DOI：
10.4067/s0718-58392016000300004
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发表时间：
2016-09-01
影响因子：
1.7
通讯作者：
Avci, Fatma
中科院分区：
文献类型：
--
作者：
Kahriman, Fatih;Egesel, Cem Ömer;Avci, Fatma
在现有文献中，图形技术已成为展示玉米育种实验结果的重要工具。本研究比较了不同的图解技术，以确定玉米产量和籽粒品质性状的最佳亲本系和杂交组合。我们在5 × 2线x测试仪设计中测量了单株产量、蛋白质含量、油含量、类胡萝卜素含量、油酸和亚油酸。采用基因型+基因型x环境（GE）双图、主成分分析（PCA）双图和极坐标图对数据进行分析，并与传统的线x检验方法进行比较。在常规分析中，一般配合力（GCA）值较高的亲本单株产量为A680和HYA，蛋白质含量为IHP，含油量为IHO和HYA，类胡萝卜素含量为A680和Q2，油酸含量为IHP，亚油酸含量为A680。B73测试器对大多数被测性状的GCA值均为正。在单株产量、蛋白质含量和含油量方面，HYA与B73组合表现最佳。结果表明，双标图方法既有优点，也有缺点。PCA双图可以单独使用，而GGE双图和极坐标图都可以用于配合力、杂种优势和基因作用分析。总体而言，图形分析结果与常规分析非常相似。因此，假设所使用的图形方法可以用于分析/呈现用x线测试设计进行的玉米育种实验的数据。
Graphical techniques have become important tools to show results of maize (Zea mays L.) breeding experiments in current literature. The present study compared different graphical techniques to determine the best parental lines and cross combinations for yield and kernel quality traits in maize breeding experiments. We measured single plant yield, protein content, oil content, carotenoid content, oleic acid, and linoleic acid in a 5 x 2 line x tester design. Genotype + genotype x environment (GE) biplot, principal component analysis (PCA) biplot, and polar plot were used to analyze data and compare them with conventional line x tester analysis. In the conventional analysis, parents with high means and positive general combining ability (GCA) values were A680 and HYA for single plant yield, IHP for protein content, IHO and HYA for oil content, A680 and Q2 for carotenoid content, IHP for oleic acid content, and A680 for linoleic acid content. The B73 tester exhibited positive GCA values for most investigated traits. The HYA x B73 combination was the best cross in terms of single plant yield, protein, and oil contents. Results showed that biplot methods had both advantages and disadvantages. The PCA biplots can be used alone while the GGE biplot and polar plots are both useful for combining ability, heterosis, and gene action analysis in a line x tester design. Overall, graphical analysis results were very similar to conventional analysis. Consequently, it was assumed that the graphical methods used could be useful to analyze/present data from maize breeding experiments carried out with a line x tester design.