Feasibility analysis of Arabidopsis thaliana as an alternative host for research on interactions of pinewood nematodes with plants
Feasibility analysis of Arabidopsis thaliana as an alternative host for research on interactions of pinewood nematodes with plants
复制标题
拟南芥作为松材线虫与植物相互作用研究替代宿主的可行性分析
DOI：
10.1007/s13313-012-0175-x
复制
发表时间：
2013-01-01
影响因子：
1.4
通讯作者：
Liu, Qian
中科院分区：
文献类型：
--
作者：
Zhao, Hai-juan;Jian, Heng;Liu, Qian
松材线虫病是引起松树枯萎病的主要病害，造成严重的经济损失。然而，由于松属植物作为寄主植物的研究还存在一定的局限性，有关B.木麻黄和松树在分子水平上的作用有限。使用替代植物将大大有助于更好地了解植物与线虫的相互作用。本研究探索了拟南芥作为替代宿主来研究植物与B关系的可行性。并建立了A. B.木蠹蛾（定义为受B侵染的平均植株数。采用人工伤苗法、苗龄法和线虫接种浓度法测定了9种松材线虫的敏感性。测试了thaliana生态型。结果表明，B.木虱侵染所有供试生态型。伤害第一叶叶柄可提高B的植株侵染率。接种浓度为400条线虫/株的接种植株在发芽后15天（DAG）达到90%。典型的症状是接种后72 h整株黄化和老叶布朗宁。类囊体膜的膜损伤、叶绿体的崩解和细胞质电子密度的增加。结果表明，B.木虱能入侵拟南芥并引起与松树相似的早期症状，而B. xylophilus不能在A. thaliana.尽管如此，A.拟南芥可用于研究植物对B的早期反应。为研究B提供了新的契机。木生植物相互作用
Bursaphelenchus xylophilus causes pine wilt disease and substantial economic losses. However, as Pinus has some limitations as a host for studies, information about the interactions between B. xylophilus and Pinus at the molecular level is limited. The use of an alternative plant would greatly facilitate a better understanding of the plant-nematode interactions. In this study, we explored the feasibility of Arabidopsis thaliana as an alternative host to investigate the relationship between plants and B. xylophilus and established a system for the efficient infection of A. thaliana by B. xylophilus (defined in this paper as the mean number of plants infested with B. xylophilus) by evaluating the artificial wounding of seedlings, ages of seedlings and nematode inoculum concentrations, and then the susceptibility of nine A. thaliana ecotypes was tested. The results showed that B. xylophilus infected all ecotypes tested. Artificially wounding the petiole of the first leaf increased the plant infection rate of B. xylophilus, reaching 90 % of the inoculated plants with inoculum concentration of 400 nematodes per plant at 15 days after germination (DAG). A typical symptom was yellowing of the entire plant and browning of older leaves 72 h after inoculation. Membrane damage of the thylakoid lamellae, collapse of chloroplasts and increase of the electron density of the cytoplasm were also observed. Our results demonstrated that B. xylophilus could invade Arabidopsis and cause similar early symptoms to that of pine trees, yet B. xylophilus could not reproduce in A. thaliana. Nonetheless, A. thaliana may be used to study the early response of plants to B. xylophilus and it provides a new opportunity for studying B. xylophilus-plant interactions.