Rapid effects of dorsal hippocampal G-protein coupled estrogen receptor on learning in female mice
Rapid effects of dorsal hippocampal G-protein coupled estrogen receptor on learning in female mice
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DOI：
10.1016/j.psyneuen.2016.11.019
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发表时间：
2017-03-01
影响因子：
3.7
通讯作者：
Choleris, Elena
中科院分区：
文献类型：
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作者：
Lymer, Jennifer;Robinson, Alana;Choleris, Elena
通过快速的作用机制，雌激素影响学习和记忆过程。研究表明，17 - β -雌二醇和雌激素受体(ER) α激动剂在系统给药或注入海马背侧时，可以提高社会识别、物体识别和物体放置任务的表现。相反，全身和背侧海马内质网β激活仅促进空间学习。此外，17 β -雌二醇、ER α和g蛋白偶联雌激素受体（GPER）激动剂可增加CA1海马的树突棘密度。最近，我们发现GPER的选择性系统激活也迅速促进了雌性小鼠的社会识别、物体识别和物体放置学习。特异性激活海马背侧GPER是否也能快速改善习得前的学习和记忆尚不清楚。在此，我们研究了GPER激动剂G-1（剂量：50 nM、100 nM、200 nM）在海马背侧输注对社会识别、物体识别和物体放置学习任务的快速影响。这些范式在40分钟内完成，在快速雌激素效应的范围内。海马背侧给药G-1改善了社会识别（剂量：50 nM， 200 nM G-1）和物体识别（剂量：200 nM G-1），但对物体放置没有影响。此外，当空间线索在y -仪器中被最小化时，G-1给药促进了社会（剂量：100 nM、200 nM G-1）和物体（剂量：50 nM、100 nM、200 nM G-1）识别。因此，与内质网α一样，海马体中的GPER似乎足以快速促进雌性小鼠的社会和物体识别，但不足以快速促进物体放置学习。因此，海马背侧的GPER参与了雌激素介导的学习和记忆，这些作用可能是通过快速信号传导机制发生的。(C) 2016 Elsevier Ltd.版权所有。
Through rapid mechanisms of action, estrogens affect learning and memory processes. It has been shown that 17 beta-estradiol and an Estrogen Receptor (ER) alpha agonist enhances performance in social recognition, object recognition, and object placement tasks when administered systemically or infused in the dorsal hippocampus. In contrast, systemic and dorsal hippocampal ER beta activation only promote spatial learning. In addition, 17 beta-estradiol, the ER alpha and the G-protein coupled estrogen receptor (GPER) agonists increase dendritic spine density in the CA1 hippocampus. Recently, we have shown that selective systemic activation of the GPER also rapidly facilitated social recognition, object recognition, and object placement learning in female mice. Whether activation the GPER specifically in the dorsal hippocampus can also rapidly improve learning and memory prior to acquisition is unknown. Here, we investigated the rapid effects of infusion of the GPER agonist, G-1 (dose: 50 nM, 100 nM, 200 nM), in the dorsal hippocampus on social recognition, object recognition, and object placement learning tasks in home cage. These paradigms were completed within 40 min, which is within the range of rapid estrogenic effects. Dorsal hippocampal administration of G-1 improved social (doses: 50 nM, 200 nM G-1) and object (dose: 200 nM G-1) recognition with no effect on object placement. Additionally, when spatial cues were minimized by testing in a Y-apparatus, G-1 administration promoted social (doses: 100 nM, 200 nM G-1) and object (doses: 50 nM, 100 nM, 200 nM G-1) recognition. Therefore, like ER alpha, the GPER in the hippocampus appears to be sufficient for the rapid facilitation of social and object recognition in female mice, but not for the rapid facilitation of object placement learning. Thus, the GPER in the dorsal hippocampus is involved in estrogenic mediation of learning and memory and these effects likely occur through rapid signalling mechanisms. (C) 2016 Elsevier Ltd. All rights reserved.