Application of SSR Markers for Purity Testing of Commercial Hybrid Soybean ( Glycine max L.)
Application of SSR Markers for Purity Testing of Commercial Hybrid Soybean ( Glycine max L.)
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DOI：
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发表时间：
2014-11
影响因子：
1.2
通讯作者：
C. B. Zhang;B. Peng;W. L. Zhang;S. M. Wang;H. Sun;Y. Dong;L. Zhao
中科院分区：
文献类型：
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作者：
C. B. Zhang;B. Peng;W. L. Zhang;S. M. Wang;H. Sun;Y. Dong;L. Zhao
要充分利用任何杂交种的潜力，都需要拥有基因纯度高的种子。利用细胞质雄性不育（CMS）系统开发了商品大豆杂交品种。为了避免使用低纯度种子导致产量下降，开发一种简单、快速、准确的杂交纯度评估方法具有重要的意义。因此，利用160个SSR标记对HybSoy 1 ~ 5亲本进行了筛选，其中8个标记具有多态性。用这些标记进行的pcr检测检测到纯杂交种亲本系的两个等位基因，证明了它们的杂合性，而杂质仅通过一个亲本等位基因的存在来鉴定。杂种纯度的证实表明，一个多态性标记足以检测这些杂种来自其亲本的污染。如果一个杂交种子批次被另一个杂交或其亲本系污染，可以使用两个或更多合适的标记来容易地检测这种污染。该方法可以准确有效地鉴定由亲本系种子混交而成的大豆杂种的纯度。这是首次报道利用SSR标记评价大豆杂交品种的遗传纯度。
Exploitation of the full potential of any hybrid requires the possessing of genetically high-purity seeds. Commercial soybean hybrids have been developed using a cytoplasmic male sterility (CMS) system. In order to avoid reduction in yield caused by using lowpurity seeds, development of a simple, rapid, and accurate method for hybrid purity assessment is of great essence and significance. Therefore, the parental lines of HybSoy 1 to 5 were screened using 160 Simple Sequence Repeat (SSR) makers, of which 8 markers exhibited polymorphism. A PCR-based assay with these markers detected both alleles of the parental lines in pure hybrids, proving their heterozygosity, whereas impurities were identified by the presence of only one parental allele. The confirmation of hybrid purity indicated that a single polymorphic marker was sufficient for detection of contaminations of these hybrids from their parents. It was also found that if a hybrid seed lot was contaminated by another hybrid or its parental lines, two or more appropriate markers could be used to easily detect such contamination. This method could accurately and effectively identify the hybrid purity in a predetermined sample of soybean hybrids constituted by deliberately mixing seeds of parental lines. This is the first report that demonstrates the utility of SSR markers for assessment of genetic purity of soybean hybrids.