Hepatitis C virus complete genome sequences identified from China representing subtypes 6k and 6n and a novel, as yet unassigned subtype within genotype 6
Hepatitis C virus complete genome sequences identified from China representing subtypes 6k and 6n and a novel, as yet unassigned subtype within genotype 6
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DOI：
10.1099/vir.0.81400-0
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发表时间：
2006-03-01
影响因子：
3.8
通讯作者：
Hagedorn, CH
中科院分区：
文献类型：
--
作者：
Lu, L;Nakano, T;Hagedorn, CH
本文报道了中国分离的3种丙型肝炎病毒变异株km41、km42和gz52557的全基因组序列。它们的基因组全长分别为9430、9441和9448个核苷酸，5‘非翻译区分别为341、342和339个核苷酸，其次是9045、9045和9057个核苷酸，3’非翻译区分别为41、51和52个核苷酸。系统发育分析表明，km41属于6k亚型，km42属于6n亚型。虽然gz52557与6g亚型聚在一起，但似乎属于一个不同的亚型。通过对分别代表17个6a~6q亚型的53个和105个NS5B区序列的联合分析，发现gz52557与所有这些分离物的距离相等，属于一个新的亚型。然而，基于最近的共识，即新的丙型肝炎病毒亚型指定需要三个或更多的例子，建议gz52557仍然没有被分配到任何亚型。
Here, the complete genome sequences for three hepatitis C virus (HCV) variants identified from China and belonging to genotype 6 are reported: km41, km42 and gz52557. Their entire genome lengths were 9430, 9441 and 9448 nt, respectively; the 5' untranslated regions (UTRs) contained 341, 342 and 339 nt, followed by single open reading frames of 9045, 9045 and 9057 nt, respectively; the 3' UTRs, up to the poly(U) tracts, were 41, 51 and 52 nt, respectively. Phylogenetic analyses showed that km41 is classified into subtype 6k and km42 into subtype 6n. Although gz52557 clustered distantly with subtype 6g, it appeared to belong to a distinct subtype. Analysis with 53 and 105 partial core and NS5B region sequences, respectively, representing 17 subtypes from 6a to 6q and three unassigned isolates of genotype 6 in co-analyses demonstrated that gz52557 was equidistant from all of these isolates, indicating that it belongs to a novel subtype. However, based on a recent consensus that three or more examples are required for a new HCV subtype designation, it is suggested that gz52557 remains unassigned to any subtype.