Mixed Phenolic Acids Mediated Proliferation of Pathogens Talaromyces helicus and Kosakonia sacchari in Continuously Monocultured Radix pseudostellariae Rhizosphere Soil
Mixed Phenolic Acids Mediated Proliferation of Pathogens Talaromyces helicus and Kosakonia sacchari in Continuously Monocultured Radix pseudostellariae Rhizosphere Soil
复制标题
混合酚酸介导连续单株太子参根际土壤中镰状菌和糖Kosakonia 病原体的增殖
DOI：
10.3389/fmicb.2016.00335
复制
发表时间：
2016-03-17
影响因子：
5.2
通讯作者：
Lin, Wenxiong
中科院分区：
文献类型：
--
作者：
Wu, Hongmiao;Wu, Linkun;Lin, Wenxiong
太子参是一种常用的中药。然而，连续单一栽培增加了其对严重疾病的易感性。在连续单一栽培的拟星田根际土壤中鉴定出两种致病微生物Talaromyces helicus M. （KU355274）和Kosakonia sacchari W. （KU324465）及其拮抗菌杆状芽孢杆菌Z.。在根际土壤和无菌培养基中鉴定出9种不同类型的酚酸。丁香酸和酚酸的混合物显著促进了螺旋体和糖糜酵母的生长。螺旋霉可以利用8种酚酸，而糖蜜霉只能利用4种。K. sacchari在消耗香兰素时产生原儿茶酸。原儿茶酸对小扁豆的生长有负向影响。在低浓度条件下，螺旋螺旋体产生的3A-DON毒素促进了糖酵母菌的生长，抑制了矮螺旋体的生长。这些数据有助于解释为什么酚类分泌物介导了连作伪姜根际土壤的微生物区系转移和结构紊乱，并导致再植病发病率增加。
Radix pseudostellariae L. is a common and popular Chinese medication. However, continuous monoculture has increased its susceptibility to severe diseases. We identified two pathogenic microorganisms, Talaromyces helicus M. (KU355274) and Kosakonia sacchari W. (KU324465), and their antagonistic bacterium, Bacillus pumilus Z. in rhizosphere soil of continuously monocultured R. pseudostellariae. Nine types of phenolic acids were identified both in the rhizosphere soil and in culture medium under sterile conditions. A syringic acid and phenolic acid mixture significantly promoted the growth of T. helicus and K. sacchari. T. helicus could utilize eight types of phenolic acids, whereas K. sacchari could only use four phenolic acids. K. sacchari produced protocatechuic acid when consuming vanillin. Protocatechuic acid negatively affected the growth of B. pumilus. The 3A-DON toxin produced by T. helicus promoted the growth of K. sacchari and inhibited growth of B. pumilus at low concentrations. These data help explain why phenolic exudates mediate a microflora shift and structure disorder in the rhizosphere soil of continuously monocultured R. pseudostellariae and lead to increased replanting disease incidence.