Evaluation of N-acetyltaurine as an ethanol marker in human blood
Evaluation of N-acetyltaurine as an ethanol marker in human blood
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DOI：
10.1016/j.alcohol.2017.05.007
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发表时间：
2017-12-01
期刊：
影响因子：
2.3
通讯作者：
Weinmann, Wolfgang
中科院分区：
文献类型：
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作者：
Luginbuhl, Marc;Konig, Stefan;Weinmann, Wolfgang
为了研究血液中N-乙酰牛磺酸（NAcT）作为酒精摄取生物标志物的潜力，对先前发表的尿液LC-MS/MS方法进行了修改，以同时检测NAcT和乙基葡萄糖醛酸苷（EtG）。应用该方法对147份法医物证进行了分析。在饮酒研究中，与EtG相反，NAcT被证明是一种内源性物质，在戒酒2周后血液中存在22 +/- 7 ng/mL（13-31 ng/mL）。饮酒后观察到NAcT中度增加至40 +/- 10 ng/mL（27-57 ng/mL）。在24小时内，NAcT浓度下降到起始浓度在七个八个科目。达到445 +/- 101 ng/mL（278-662 ng/mL）的峰EtG浓度（（c）/bar（最大值））。虽然血液中的EtG可用于检测酒精消耗，即使乙醇已经被消除，但单次乙醇剂量后的一些最大NAcT浓度在其他受试者开始饮酒前检测到的内源性水平范围内。在147份血液样本中，发现了以下浓度：血液酒精浓度（BAC）：1.22 +/- 0.95 g/kg（0-3.46 g/kg）; NAcT：37.8 +/- 18.4 ng/mL（12.1-109 ng/mL）; EtG：1149 +/- 1121 ng/mL（0-5950 ng/mL）。对EtG和NAcT进行0.8和1.6 g/kg BAC阈值的ROC曲线分析。由于与EtG相比，内源性NAcT水平的存在导致较低的灵敏度和选择性，并且由于酒精摄取后浓度略有增加，NAcT作为血液中酒精生物标志物的有用性非常有限。(C)2017爱思唯尔公司All rights reserved.
To investigate the potential of N-acetyltaurine (NAcT) in blood as a biomarker for alcohol uptake, a previously published LC-MS/MS method for urine was modified to simultaneously detect NAcT and ethyl glucuronide (EtG). The method was applied in a drinking study and by analyzing 147 forensic case samples. In the drinking study, contrary to EtG, NAcT proved to be an endogenous substance, which was present at 22 +/- 7 ng/mL (13-31 ng/mL) in the blood after 2 weeks of abstinence. A moderate increase in NAcT to 40 +/- 10 ng/mL (27-57 ng/mL) was observed after drinking. Within 24 h, the NAcT concentrations declined to starting concentrations in seven out of eight subjects. Peak EtG concentrations ((c) over bar (max)) of 445 +/- 101 ng/mL (278-662 ng/mL) were reached. While EtG in blood can be used to detect alcohol consumption even if ethanol is already eliminated, some of the maximum NAcT concentrations after a single ethanol dose were in the range of endogenous levels detected prior to the start of drinking in other subjects. In the 147 blood samples, the following concentrations were found: blood alcohol concentration (BAC): 1.22 +/- 0.95 g/kg (0-3.46 g/kg); NAcT: 37.8 +/- 18.4 ng/mL (12.1-109 ng/mL); EtG: 1149 +/- 1121 ng/mL (0-5950 ng/mL). ROC curve analysis for BAC thresholds at 0.8 and 1.6 g/kg were performed for EtG and NAcT. Due to the presence of endogenous NAcT levels resulting in a lower sensitivity and selectivity when compared to EtG, and due to a minor increase in concentration after alcohol uptake, the usefulness of NAcT as an alcohol biomarker in blood is very limited. (C) 2017 Elsevier Inc. All rights reserved.