AN ANIMAL-MODEL OF CONGENITAL DEFECT OF GENE-EXPRESSION OF CHOLECYSTOKININ (CCK)-A RECEPTOR
AN ANIMAL-MODEL OF CONGENITAL DEFECT OF GENE-EXPRESSION OF CHOLECYSTOKININ (CCK)-A RECEPTOR
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DOI：
10.1006/bbrc.1995.1728
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发表时间：
1995-05-25
影响因子：
3.1
通讯作者：
KONO, A
中科院分区：
文献类型：
--
作者：
FUNAKOSHI, A;MIYASAKA, K;KONO, A
应用逆转录聚合酶链反应和Southern杂交技术检测OLETF大鼠胚胎和成年胰腺中CCK-A和B受体基因的表达。在体外检测胰腺对各种刺激物的反应，并将结果与对照（LETO）大鼠的结果进行比较。CCK-A受体mRNA在两种品系的胎儿胰腺和OLETF大鼠的成年胰腺中均不表达，但在LETO大鼠的成年胰腺中表达。两种品系胎儿和成年胰腺均有CCK-B受体mRNA表达。Southern印迹杂交表明两株菌基因结构存在差异。最大有效浓度的神经介肽C，卡巴胆碱，和分泌素的淀粉酶分泌和细胞内钙离子运动刺激卡巴胆碱和神经介肽C是相似的两种菌株。CCK-8和非硫酸化形式刺激淀粉酶分泌仅在LETO大鼠。这些结果表明，OLETF大鼠是一个新的模型的先天性缺陷的CCK-A受体基因，并应用于确定CCK受体功能。(C)出版社：Academic Press
Expressions of the CCK-A and B receptor genes in fetal and adult pancreas of OLETF rats were examined by the reverse transcriptase polymerase chain reaction followed by Southern blot hybridization. The pancreatic responses to various stimulants were examined in vitro and results were compared with those of control (LETO) rats. CCK-A receptor mRNA was not expressed in the fetal pancreas of either strain or in the adult pancreas of OLETF rats, but was expressed in the adult pancreas of LETO rats. CCK-B receptor mRNA was expressed in fetal and adult pancreas in both strains. Southern blot hybridization indicated a difference in gene structure in the two strains. The maximal effective concentrations of neuromedin C, carbachol, and secretin for amylase secretion and intracellular Ca2+ movement stimulated by carbachol and neuromedin C were similar in the two strains. CCK-8 and the non-sulfated form stimulated amylase secretion only in LETO rats. These results suggest that OLETF rats are a new model of a congenital defect of the CCK-A receptor gene and should be useful for determining CCK receptor function. (C) 1995 Academic Press,Inc.