Light emission requires exposure to the atmosphere in ex vivo bioluminescence imaging
Light emission requires exposure to the atmosphere in ex vivo bioluminescence imaging
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DOI：
10.2310/7290.2006.00015
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发表时间：
2006-04-01
影响因子：
2.8
通讯作者：
Ohtomo, Kuni
中科院分区：
文献类型：
--
作者：
Inoue, Yusuke;Izawa, Kiyoko;Ohtomo, Kuni
通过体内生物发光成像(BLI)来识别具有荧光素酶活性的器官往往是困难的，而体外组织器官成像则起着补充作用。这项研究调查了在体外BLI中暴露在大气中的重要性。将转导萤火虫荧光素酶和p190 bcr-abl基因的小鼠Pro-B细胞系BA/F3接种于小鼠。在活体BLI后处死动物，死后行全身显像，然后进行腹膜腔空气注射。此外，还暴露了右膝，并在切割相邻骨骼前后进行了成像。在活体成像上可见广泛的光信号。在动物被杀后，发光消失，空气注射仅恢复了腹部的战斗发射，表明大气中的氧气在死亡后的发光中起着关键作用。虽然在截骨前右膝没有明显的光信号，但截骨后有明显的光发射。总而言之，在体外BLI中，紧密发射需要暴露在大气中。死后骨髓中的荧光素酶活性需要骨质破坏。
The identification of organs bearing luciferase activity by in vivo bioluminescence imaging (BLI) is often difficult, and ex vivo imaging of excised organs plays a complementary role. This study investigated the importance of exposure to the atmosphere in ex vivo BLI. Mice were inoculated with murine pro-B cell line Ba/F3 transduced with firefly luciferase and p190 BCR-ABL. They were killed following in vivo BLI, and wholebody imaging was done after death and then after intraperitoneal air injection. In addition, the right knee was exposed and imaged before and after the adjacent bones were cut. Extensive light signals were seen on in vivo imaging. The luminescence disappeared after the animal was killed, and air injection restored the fight emission from the abdomen only, suggesting a critical role of atmospheric oxygen in luminescence after death. Although no substantial light signal at the right knee was seen before bone cutting, light emission was evident after cutting. In conclusion, in ex vivo BLI, tight emission requires exposure to the atmosphere. Bone destruction is required to demonstrate luciferase activity in the bone marrow after death.