Equine herpesvirus 1 mutants devoid of glycoprotein B or M are apathogenic for mice but induce protection against challenge infection.
Equine herpesvirus 1 mutants devoid of glycoprotein B or M are apathogenic for mice but induce protection against challenge infection.
复制标题
缺乏糖蛋白 B 或 M 的马疱疹病毒 1 突变体对小鼠无致病性，但可诱导针对攻击感染的保护。
作者：
A. Neubauer;M. Beer;C. Brandmüller;O. Kaaden;N. Osterrieder
Equine herpesvirus 1 (EHV-1) mutants devoid of the open reading frames (ORFs) of either glycoprotein (g) B or M were constructed and tested for their immunogenic potential in a murine model of EHV-1 infection. The mutant viruses were engineered using the virulent EHV-1 strain RacL11 or the modified live vaccine strain RacH by inserting the Escherichia coli LacZ gene into the viral ORFs. RacL11-infected mice showed signs typical of an EHV-1 infection, whereas mice infected with the EHV-1 gB- or gM-negative mutants or with RacH did not develop disease. No difference in the pathogenic potential of RacL11 gB- and gM-negative viruses was observed after application of either phenotypically completed or negative viruses. However, revertant RacL11 viruses in which the gB or gM gene had been restored caused EHV-1-related symptoms that were indistinguishable from those induced by RacL11. Mice that had been immunized with phenotypically negative gB- and gM-deficient EHV-1 were challenged with the RacL11 virus 25 days after immunization. Mock-immunized mice developed EHV-1 disease and high virus loads in their lungs were observed. In contrast, mice developed not exhibit EHV-1-caused disease. It was concluded (i) that deletion of either gB or gM abolished the virulence of strain RacL11 and (ii) that immunization with gB- or gM-negative EHV-1 elicited a protective immunity that was reflected by both virus-neutralizing antibodies and EHV-1-specific T-cells in spleens of immunized mice.
胸苷激酶阴性马疱疹病毒 1 疫苗对幼马的安全性和有效性。
DOI：
--
发表时间：
1990
期刊：
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire
影响因子：
--
作者：
Cornick,J;Martens,J;Martens,R;Crandell,R;McConnell,S;Kit,S
通讯作者：
Kit,S
复制缺陷型单纯疱疹病毒 2 ICP8 突变株的构建和表征及其在豚鼠生殖器疾病模型免疫研究中的应用。
DOI：
10.1006/viro.1997.8564
发表时间：
1997
期刊：
Virology.
影响因子：
--
作者：
DaCosta,XJ;Bourne,N;Stanberry,LR;Knipe,DM
通讯作者：
Knipe,DM
马疱疹病毒1型糖蛋白14的合成和加工。
影响因子：
3.7
作者：
Sullivan,DC;Allen,GP;O'Callaghan,DJ
通讯作者：
O'Callaghan,DJ