Genome-wide analysis of cabbage cytochrome P450 genes and characterization of BoCYP704B1, a gene responsible for cabbage anther development
Genome-wide analysis of cabbage cytochrome P450 genes and characterization of BoCYP704B1, a gene responsible for cabbage anther development
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DOI：
10.1016/j.scienta.2021.110096
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发表时间：
2021-03-05
影响因子：
4.3
通讯作者：
Han, Fengqing
中科院分区：
文献类型：
--
作者：
Ji, Jialei;Cao, Wenxue;Han, Fengqing
植物细胞色素P450单加氧酶(Cyps)是一个血红素硫酸酯酶超家族，参与多种初级和次级代谢反应。本研究在甘蓝基因组中鉴定了279个甘蓝CYP(BoCYP)基因。这些BoCyp被划分为8个不同的部族，44个科。对甘蓝不同组织中BoCYP基因的表达分析表明，部分BoCYP基因可能在甘蓝生殖过程中发挥重要作用。最近，BoCYP704B1已被鉴定为甘蓝83121A雄性不育相关的候选基因。本文对BoCYP704B1的作用机制进行了探讨。亚细胞定位结果表明，BoCYP704B1位于内质网。RNA原位杂交结果表明，BoCYP704B1主要在甘蓝花药绒毡层中表达，在四分体和单核细胞早期表达最强，直至终止表达。功能互补试验结果表明，野生型BoCYP704B1可以挽救83121A突变体的雄性不育。本研究结果加深了对甘蓝细胞色素P704B1基因作用机制的认识，为进一步研究BoCYP704B1基因在甘蓝分子育种中的应用奠定了基础。
Plant cytochrome P450 monooxygenases (CYPs) make up a large superfamily of heme-thiolate enzymes involving in many primary and secondary metabolism reactions. In this study, a total of 279 Brassica oleracea CYP (BoCYP) genes were identified in the cabbage genome. These BoCYPs were classified into eight distinct clans with 44 families. Expression analyses of the BoCYP genes of different cabbage tissues suggested that some BoCYPs may play significant roles in reproduction. Recently, BoCYP704B1 has been identified as a candidate gene associated with male sterility in the cabbage line 83121A. Here, the functional mechanism of BoCYP704B1 was explored. Subcellular localization showed that BoCYP704B1 is located in the endoplasmic reticulum. RNA in situ hybridization indicated that BoCYP704B1 is predominantly expressed in the tapetum of cabbage anther, and the expression was maximal at the tetrad and early monocyte stage and weakened to the termination. Furthermore, functional complementation test results showed that BoCYP704B1 from wild type could rescue the male sterility of 83121A mutant. The results of this study increase our understanding of the functional mechanism of cabbage CYP genes and will facilitate the application of the BoCYP704B1 gene in cabbage molecular breeding.