Creative damage unleashes transcription.
Creative damage unleashes transcription.
复制标题
创造性的破坏释放了转录的力量。
DOI：
10.1080/15384101.2016.1154370
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Calderwood,StuartK
中科院分区：
文献类型：
--
作者：
Calderwood,StuartK
It is well known that DNA replication is subject to error and that mechanisms are in place to detect and repair such mistakes. Perhaps more surprisingly, recent studies have suggested a similar state of affairs with regard to transcription by RNA Polymerase II (Pol II). Transcription has been associated with the accumulation of DNA damage and tissues with high transcriptional rates such as growing tumors are at risk for increased levels of mutation. 1 In addition, some proteins involved in repair of DNA damage appear to play key parts in transcription. These include, factors involved in base excision repair, nucleotide excision repair, mismatch repair and recombination repair. 2 Here we will discuss potential mechanisms for transcription-associated DNA damage, subsequent triggering of DNA damage repair (DDR) pathways, and the influence of DDR signaling on transcription. Genomic DNA is at increased risk for damage after chromatin decondenses close to the transcriptional start site (TSS), where naked DNA, stripped of protective nucleosomal proteins may become accessible to reactive oxygen species in the cell. 3 Transcription-associated damage could also be incurred, when the DNA double helix is unwound in general transcription factor (GTF)-RNA polymerase II (Pol II) complexes on gene promoters and copied into RNA sequences. 2, 3 R loop formation may occur on initiation of transcription; in this process the growing RNA strand hybridizes with the single stranded template DNA sequence. The displaced DNA strand becomes susceptible to chemical damage and formation of secondary structures that are prone to recombination and mutation. 2 Melting of the DNA double strand by Pol II also results in torsion, generating negative supercoiling in the upstream DNA, with further potential for oxidative damage and positive supercoiling downstream that may retard Pol II processivity. In addition to such sporadic damage there appears to be a pathway of “scheduled DNA strand breakage” associated with transcription. Inducible gene transcription has been shown to be accompanied by the generation of DNA double strand breaks (DSB) in cells activated by agents as diverse as heat shock, growth serum, androgens, neuronal activation or estrogens. 1, 4-7 Increased DSB have been detected directly in transcribing cells, as well as indirectly by the Comet assay and by incorporation into chromatin of the histone gH2Ax, a surrogate marker of DNA damage. 5-7 Indeed direct generation of DSB in the promoters of the EGR1 and NPAS4 genes, using a CRISPR-CAS approach, was sufficient to drive transcription even in the absence of external stimuli. 7 Repair of DSBs requires DNA recombination repair by mechanisms including homologous recombination (HR) and non-homologous end joining repair (NHEJ). 2 It has been shown that some of the components of the NHEJ pathway such as DNA-dependent kinase (DNA-PK), Ku70 and Ku80 become associated with activated genes, including estrogen-induced genes, heat shock genes and serum-inducible immediate early (IE) genes. 4, 6 The NHEJ intermediates were shown to be associated with ER-induced genes in a complex with Poly (ADP-Ribose) Polymerase 1 (PARP1), Topoisomerase IIb and transcription appeared to require Topoisomerase IIb-mediated nicking of sequences within the promoter of the presenilin 2 (pS2) gene 4 (Fig. 1). In fact a role for Topoisomerase IIb in transcriptional activation appeared to be a general finding in a number of the studies. 4, 6, 7 It is not clear which exact stimulus provokes Topoisomerase IIb to bind and induce DSBs in transcribing genes, although direct recruitment of …
