Amphibacillus xylanus gen. nov., sp. nov., a Facultatively Anaerobic Sporeforming Xylan-Digesting Bacterium Which Lacks Cytochrome, Quinone, and Catalase
Amphibacillus xylanus gen. nov., sp. nov., a Facultatively Anaerobic Sporeforming Xylan-Digesting Bacterium Which Lacks Cytochrome, Quinone, and Catalase
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木聚糖两栖芽孢杆菌属
DOI：
10.1099/00207713-41-1-190
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发表时间：
1990
影响因子：
2.8
通讯作者：
M. Kozaki
中科院分区：
文献类型：
--
作者：
Y. Niimura;E. Koh;F. Yanagida;Ken;K. Komagata;M. Kozaki
从堆肥中分离到3株革兰氏阳性、兼性厌氧、芽胞形成、杆状细菌。这些微生物在碱性介质中生长良好，在以柠檬酸钛为还原剂的严格厌氧培养和摇动好氧培养中都能很好地消化木聚糖。细胞内含有中位二氨基甲酸，其细胞脂肪酸由异支酸、正支酸和相当数量的直链酸组成。这些菌株的DNA碱基组成范围为36-38摩尔%鸟嘌呤+胞嘧啶。未检测到细胞色素、类异戊二烯和过氧化氢酶活性。DNA-DNA同源性测定未显示与芽孢杆菌属、梭状芽孢杆菌属和芽胞乳杆菌属代表种的菌株的亲缘关系。考虑到其特性的独特性、5S rRNA的序列以及独特的代谢途径，我们提出了木质双歧杆菌基因。Nov.，sp.11月，对这些菌株。模式毒株为EP01(=JCM 7361)。
Three strains of gram-positive, facultatively anaerobic, sporeforming, rod-shaped bacteria were isolated from composts of manure with grass and rice straw. These organisms grew well in an alkaline medium and digested xylan both in strictly anaerobic cultures when titanium(III) citrate was used as a reducing agent and in aerobic cultures with shaking. The cells contained meso-diaminopimelic acid, and their cellular fatty acids consisted of iso- and anteiso-branched acids and considerable amounts of straight-chain acids. The DNA base composition of these strains ranged from 36 to 38 mol% guanine plus cytosine. Cytochromes, isoprenoid quinones, and catalase activity were not detected. DNA-DNA homology determinations did not show relatedness to strains of representative species of the genera Bacillus, Clostridium, and Sporolactobacillus. Considering the uniqueness of the characteristics, the sequence of the 5S rRNA, and the unique metabolic pathways, we propose Amphibacillus xylanus gen. nov., sp. nov., for these strains. The type strain is strain Ep01 (= JCM 7361).