A microfluidic platform integrating functional vascularized organoids-on-chip
A microfluidic platform integrating functional vascularized organoids-on-chip
复制标题
集成功能性血管化类器官芯片的微流控平台
DOI：
10.1038/s41467-024-45710-4
复制
发表时间：
2024
影响因子：
16.6
通讯作者：
Quintard C
中科院分区：
文献类型：
--
作者：
Quintard C
微流控芯片中血管网络的发育对于三维细胞聚集体如球状体、类器官、类肿瘤或组织外植体的长期培养至关重要。尽管微血管网络系统和类器官技术的快速发展，血管化芯片上的类器官仍然是组织工程中的一个挑战。大多数现有的微流体装置不能很好地反映体内流动的复杂性，并且需要复杂的技术设置。考虑到这些限制，我们开发了一个平台来建立和监测间充质和胰岛球状体周围内皮网络的形成，以及由多能干细胞产生的血管类器官，在芯片上培养长达30天。我们发现，这些网络与富含内皮的球体和血管类器官建立了功能性连接，因为它们成功地为这些结构提供了血管内灌注。我们发现，当使用我们的血管化方法在芯片上培养时，类器官的生长、成熟和功能得到增强。这种微生理系统代表了一种可行的器官芯片模型，可以使各种生物3D组织血管化，并为使用先进的微流体技术建立类器官灌注奠定了基础。
The development of vascular networks in microfluidic chips is crucial for the long-term culture of three-dimensional cell aggregates such as spheroids, organoids, tumoroids, or tissue explants. Despite rapid advancement in microvascular network systems and organoid technologies, vascularizing organoids-on-chips remains a challenge in tissue engineering. Most existing microfluidic devices poorly reflect the complexity of in vivo flows and require complex technical set-ups. Considering these constraints, we develop a platform to establish and monitor the formation of endothelial networks around mesenchymal and pancreatic islet spheroids, as well as blood vessel organoids generated from pluripotent stem cells, cultured for up to 30 days on-chip. We show that these networks establish functional connections with the endothelium-rich spheroids and vascular organoids, as they successfully provide intravascular perfusion to these structures. We find that organoid growth, maturation, and function are enhanced when cultured on-chip using our vascularization method. This microphysiological system represents a viable organ-on-chip model to vascularize diverse biological 3D tissues and sets the stage to establish organoid perfusions using advanced microfluidics.
在高通量的体外平台中灌注3D血管生成。
影响因子：
9.8
作者：
van Duinen V;Zhu D;Ramakers C;van Zonneveld AJ;Vulto P;Hankemeier T
通讯作者：
Hankemeier T
影响因子：
14.8
作者：
Wimmer, Reiner A.;Leopoldi, Alexandra;Penninger, Josef M.
通讯作者：
Penninger, Josef M.
影响因子：
17.1
作者：
Paek, Jungwook;Park, Sunghee E.;Huh, Dongeun
通讯作者：
Huh, Dongeun