Temperature acclimation of photosynthesis in spinach leaves: analyses of photosynthetic components and temperature dependencies of photosynthetic partial reactions
Temperature acclimation of photosynthesis in spinach leaves: analyses of photosynthetic components and temperature dependencies of photosynthetic partial reactions
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DOI：
10.1111/j.1365-3040.2004.01299.x
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发表时间：
2005-04
影响因子：
7.3
通讯作者：
W. Yamori;K. Noguchi;I. Terashima
中科院分区：
文献类型：
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作者：
W. Yamori;K. Noguchi;I. Terashima
菠菜（Spinacia oleracea）植物在15/10°C （LT）或30/25°C （HT）的昼夜温度制度下生长。当样品叶片处于特定的发育阶段时，植株也会从高温转移到低温（hl转移）。在叶片完全成熟的情况下，获得了环境CO2浓度（Ca）为1500µL L−1 （A1500）时的光饱和光合速率(A)，以及低Ci区（IS）时A与细胞间CO2浓度（Ci）的初始斜率，以评估RuBP的再生能力和羧化能力。光合成分包括Rubisco和细胞色素f （Cyt f）也被测定。高温叶片中Ca浓度为360µL L−1 （A360）、A1500和IS的最适温度分别为27、36和24℃，低温叶片中Ca浓度为18、30和18℃。随着低温处理持续时间的增加，热转移叶片的最适温度逐渐接近低温处理叶片的最适温度，其中IS处理比A1500处理的最适温度变化更大、更快。通过hl转移，A1500和IS的最大值也增加了。最大A1500和Cyt - f含量比IS和Rubisco含量增加更快。Cyt f/Rubisco比值的变化反映在A1500/IS比值上。综上所述，菠菜叶片对低温的光合驯化不仅是由于RuBP再生和羧化绝对速率平衡的变化，而且还由于RuBP羧化的最适温度发生了较大变化。
Spinach (Spinacia oleracea) plants were grown under the day/night temperature regime of 15/10 °C (LT) or 30/25 °C (HT). The plants were also transferred from HT to LT when the sample leaves were at particular developmental stages (HL-transfer). With fully mature leaves, the light-saturated photosynthetic rate (A) at the ambient CO2 concentration (Ca) of 1500 µL L−1 (A1500) and the initial slope of A versus intercellular CO2 concentration (Ci) at low Ci region (IS) were obtained to assess capacities of RuBP regeneration and carboxylation. Photosynthetic components including Rubisco and cytochrome f (Cyt f) were also determined. The optimum temperatures for A at Ca of 360 µL L−1 (A360), A1500 and IS in HT leaves were 27, 36 and 24 °C, whereas those in LT leaves were 18, 30 and 18 °C. The optimum temperatures in HL-transfer leaves approached those of LT leaves with the increase in the duration at LT. The shift in the optimum temperature was greater and quicker for IS than A1500. By the HL-transfer, the maximum values of A1500 and IS also increased. The maximum A1500 and Cyt f content increased more promptly than IS and Rubisco content. Changes in the Cyt f/Rubisco ratio were reflected to those in the A1500/IS ratio. Taken together, photosynthetic acclimation to low temperature in spinach leaves was due not only to the change in the balance of the absolute rates of RuBP regeneration and carboxylation but also to the large change in the optimum temperature of RuBP carboxylation.