Auditory Fear Conditioning Alters Sensitivity of the Medial Prefrontal Cortex but this is not based on Frequency-dependent Integration
Auditory Fear Conditioning Alters Sensitivity of the Medial Prefrontal Cortex but this is not based on Frequency-dependent Integration
复制标题
听觉恐惧条件改变了内侧前额叶皮层的敏感性，但这并不是基于频率依赖的整合
DOI：
10.1016/j.neuroscience.2020.05.038
复制
发表时间：
2020-08-21
期刊：
影响因子：
3.3
通讯作者：
Xiao，Zhongju
中科院分区：
文献类型：
--
作者：
Xie，Haiting;Wu，Duobin;Xiao，Zhongju
虽然许多研究表明前额叶皮层的边缘前区（prelimbic，PL）参与了条件冻结行为的形成，但很少有人考虑PL皮层的声反应特征。重要的是，条件性恐惧学习后PL皮层听觉反应特征的变化在很大程度上是未知的。在这里，我们使用了在清醒状态下靶向mPFC的体内细胞附着记录。我们证实了成年C57小鼠的mPFC具有对清醒状态下的噪声和音调做出反应的神经元，特别是在PL皮质中。有趣的是，数据还证实了这些神经元对声音强度反应良好，但不具有频率拓扑分布特征。此外，我们还发现，听觉恐惧条件化后，PL皮层c-fos阳性神经元数量显著增加。条件性恐惧诱发的局部场电位记录和在体细胞贴附记录表明，条件性恐惧获得后，PL皮层对听觉条件刺激更加敏感。对噪声有反应的神经元比例显著增加，棘波的信噪比也增加。这些数据表明，PL神经元本身响应的主要信息（声音强度），而次要信息（频率）的反应几乎可以忽略不计后，听觉恐惧条件反射。这种现象可能是处理这种情绪记忆的功能基础，这种反应特征被认为是情绪敏感化，但不会改变这种反应的性质。
Although many studies have shown that the prelimbic (PL) cortex of the mPFC is involved in the formation of conditioned freezing behavior, few have considered the acoustic response characteristics of PL cortex. Importantly, the change in auditory response characteristics of the PL cortex after conditional fear learning is largely unknown. Here we used in vivo cell-attached recordings targeting the mPFC during the waking state. We confirmed that the mPFC of adult C57 mice have neurons that respond to noise and tone in the waking state, especially in the PL cortex. Interestingly, the data also confirmed that these neurons responded well to the intensity of sound but did not have frequency topological distribution characteristics. Furthermore, we found that the number of c-fos positive neurons in the PL cortex increased significantly after auditory fear conditioning. The auditory-induced local field potential recordings and in vivo cell-attached recordings demonstrated that the PL cortex was more sensitive to the auditory conditioned stimulus after the acquisition of conditioned fear. The proportion of neurons responding to noise was significantly increased, and the signal to noise ratio of the spikes were also increased. These data reveal that PL neurons themselves responded to the main information (sound intensity), while the secondary information (frequency) response was almost negligible after auditory fear conditioning. This phenomenon may be the functional basis for handling this type of emotional memory, and this response characteristic is thought to be emotional sensitization but does not change the nature of this response.