β1 integrins control the formation of cell chains in the adult rostral migratory stream
β1 integrins control the formation of cell chains in the adult rostral migratory stream
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DOI：
10.1523/jneurosci.2991-06.2007
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发表时间：
2007-03-07
影响因子：
5.3
通讯作者：
Muller, Ulrich
中科院分区：
文献类型：
--
作者：
Belvindrah, Richard;Hankel, Sabine;Muller, Ulrich
侧脑室室下区(SVZ)是成人脑内神经发生的主要部位。出生在SVZ的神经母细胞沿着吻端迁移流(RMS)以链的形式迁移到嗅球。关于神经母细胞在迁移过程中控制相互作用的机制，人们知之甚少。在这里，我们显示迁移的神经母细胞表达β1整合素，整合素配体层粘连蛋白定位于细胞链。利用转基因小鼠和SVZ外植体的延时录像，我们证明了β1整合素和层粘连蛋白促进细胞链的形成。层粘连蛋白还可以诱导纯化的神经母细胞聚集。我们得出结论，RMS中细胞链的形成部分是由β1整合素通过与层粘连蛋白结合来控制的。此外，我们提供的证据表明，β1类整合素是维持神经胶质管所必需的，神经胶质管中的缺陷会导致神经母细胞异位迁移到周围组织。
The subventricular zone ( SVZ) of the lateral ventricle is the major site of neurogenesis in the adult brain. Neuroblasts that are born in the SVZ migrate as chains along the rostral migratory stream ( RMS) to the olfactory bulb. Little is known about the mechanisms that control interactions between neuroblasts during their migration. Here we show that migrating neuroblasts express beta 1integrins and that the integrin ligand laminin is localized to cell chains. Using genetically modified mice and time- lapse video recordings of SVZ explants, we demonstrate that beta 1 integrins and laminin promote the formation of cell chains. Laminin also induces the aggregation of purified neuroblasts. We conclude that the formation of cell chains in the RMS is controlled in part by beta 1 integrins via binding to laminin. In addition, we provide evidence that beta 1 class integrins are required for the maintenance of the glial tubes and that defects in the glial tubes lead to the ectopic migration of neuroblasts into the surrounding tissue.