Biodegradation of dibutyl phthalate and di-(2-ethylhexyl) phthalate and microbial community changes in mangrove sediment.
Biodegradation of dibutyl phthalate and di-(2-ethylhexyl) phthalate and microbial community changes in mangrove sediment.
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DOI：
10.1016/j.jhazmat.2010.08.116
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发表时间：
2010-12
影响因子：
13.6
通讯作者：
S. Yuan;I-Chun Huang;B. Chang
中科院分区：
文献类型：
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作者：
S. Yuan;I-Chun Huang;B. Chang
本研究以台湾淡水河沿着5个采样点的红树林沉积物为研究对象，探讨邻苯二甲酸酯类化合物（PAEs）邻苯二甲酸二丁酯（DBP）和邻苯二甲酸二（2-乙基己基）酯（DEHP）的微生物降解及微生物群落的变化。DBP和DEHP的好氧降解半衰期（t1/2）分别为1.6 - 2.9 d和5.0 - 8.3 d。添加酵母膏（5 mg/L）、过氧化氢（1 mg/L）、brij 35（91μM）、腐殖酸（0.5g/L）、纤维素（0.96mg/L）和氯化钠（1%）均能促进PAE的好氧降解。沉积物样品被分离成不同粒径的组分，粒径范围从0.1-0.45到500-2000μm。具有较小粒径的沉积物组分表现出较高的PAE生物降解速率。从红树林沉积物中分离到的微生物菌株中，J2、J 4和J8菌株（均鉴定为芽孢杆菌属（Bacillus sp.））表现出最好的生物降解能力。结果表明，芽孢杆菌是红树林沉积物中PAE好氧降解的优势菌。
In this study, we investigated the microbial degradation of the phthalate esters (PAEs) dibutyl phthalate (DBP) and di-(2-ethylhexyl) phthalate (DEHP), and change in microbial communities in mangrove sediment collected from 5 sampling sites along the Tanshui River in Taiwan. Aerobic degradation half-lives (t1/2) of DBP and DEHP ranged from 1.6 to 2.9d and 5.0 to 8.3d, respectively. The addition of yeast extract (5mg/L), hydrogen peroxide (1mg/L), brij 35 (91μM), humic acid (0.5g/L), cellulose (0.96mg/L), and sodium chloride (1%) enhanced PAE aerobic degradation. Sediment samples were separated into fractions with various particle size ranges from 0.1–0.45 to 500–2000μm. Sediment fractions with smaller particle sizes demonstrated higher PAE biodegradation rates. Of the microorganism strains isolated from the mangrove sediment, strains J2, J4, and J8 (all identified as Bacillus sp.) expressed the best biodegrading ability. Our results showed that Bacillus sp. was the dominant bacteria in the process of PAE aerobic degradation in the mangrove sediments.